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Literature summary for 3.1.1.88 extracted from

  • Zhai, Y.; Li, K.; Song, J.; Shi, Y.; Yan, Y.
    Molecular cloning, purification and biochemical characterization of a novel pyrethroid-hydrolyzing carboxylesterase gene from Ochrobactrum anthropi YZ-1 (2012), J. Hazard. Mater., 221-222, 206-212.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Tween-20
-
Brucella anthropi
Tween-80
-
Brucella anthropi

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Brucella anthropi

Inhibitors

Inhibitors Comment Organism Structure
DEPC
-
Brucella anthropi
PMSF
-
Brucella anthropi
SDS
-
Brucella anthropi

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+ decreases activity Brucella anthropi
Hg2+ decreases activity Brucella anthropi
Mg2+ increases activity Brucella anthropi
Zn2+ decreases activity Brucella anthropi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
SDS-PAGE Brucella anthropi

Organism

Organism UniProt Comment Textmining
Brucella anthropi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Brucella anthropi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-cyfluthrin + H2O
-
Brucella anthropi ?
-
?
beta-cypermethrin + H2O
-
Brucella anthropi ?
-
?
deltamethrin + H2O
-
Brucella anthropi ?
-
?
lambda-cyhalothrin + H2O
-
Brucella anthropi ?
-
?
permethrin + H2O
-
Brucella anthropi ?
-
?

Synonyms

Synonyms Comment Organism
pyrethroid-hydrolyzing carboxylesterase
-
Brucella anthropi
pytZ
-
Brucella anthropi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Brucella anthropi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
56% resdidual activity remained Brucella anthropi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Brucella anthropi

pH Range

pH Minimum pH Maximum Comment Organism
5 9
-
Brucella anthropi