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Literature summary for 3.1.1.88 extracted from

  • Wang, B.Z.; Guo, P.; Hang, B.J.; Li, L.; He, J.; Li, S.P.
    Cloning of a novel pyrethroid-hydrolyzing carboxylesterase gene from Sphingobium sp. strain JZ-1 and characterization of the gene product (2009), Appl. Environ. Microbiol., 75, 5496-5500.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.88

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Sphingobium sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 0.5 mM, strong inhibition Sphingobium sp.
Cu2+ 0.5 mM, strong inhibition Sphingobium sp.
diethylpyrocarbonate 0.5 mM, strong inhibition Sphingobium sp.
Hg2+ 0.5 mM, strong inhibition Sphingobium sp.
iodoacetamide 0.5 mM, strong inhibition Sphingobium sp.
additional information 10 mM EDTA or 10 mM 1,10-phenanthroline have little effect on the enzyme activity (less than 10% inhibition) Sphingobium sp.
Ni2+ 0.5 mM, strong inhibition Sphingobium sp.
p-chloromercuribenzoate 0.5 mM, strong inhibition Sphingobium sp.
PMSF 0.5 mM, strong inhibition Sphingobium sp.
SDS 10 mM, strong inhibition Sphingobium sp.
Triton X-100 10 mM, 20-30% inhibition Sphingobium sp.
Tween 80 10 mM, strong inhibition Sphingobium sp.
Zn2+ 0.5 mM, strong inhibition Sphingobium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000062
-
 trans-permethrin pH 7.5, 37°C Sphingobium sp.
0.000065
-
 cis-permethrin pH 7.5, 37°C Sphingobium sp.
0.000106
-
 (alphaR)-fenpropathrin pH 7.5, 37°C Sphingobium sp.
0.000106
-
 (alphaS)-fenpropathrin pH 7.5, 37°C Sphingobium sp.
0.000108
-
 cis-cypermethrin pH 7.5, 37°C Sphingobium sp.
0.00011
-
 trans-cypermethrin pH 7.5, 37°C Sphingobium sp.
0.000348
-
 cyhalothrin pH 7.5, 37°C Sphingobium sp.
0.000585
-
 (alphaS,2R)-fenvalerate pH 7.5, 37°C Sphingobium sp.
0.000585
-
 (alphaS,2S)-fenvalerate pH 7.5, 37°C Sphingobium sp.
0.000585
-
 (alphaR,2R)-fenvalerate pH 7.5, 37°C Sphingobium sp.
0.000585
-
 (alphaR,2S)-fenvalerate pH 7.5, 37°C Sphingobium sp.
0.000788
-
 deltamethrin pH 7.5, 37°C Sphingobium sp.
0.00159
-
 bifenthrin pH 7.5, 37°C Sphingobium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30835
-
 1 * 30835, calculated from sequence Sphingobium sp.
31000
-
 gel filtration Sphingobium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Sphingobium sp. the enzyme is involved in degradation of pyrethroids ?
-
 ?

Organism

Organism UniProt Comment Textmining
Sphingobium sp. C0LA90 strain JZ-1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sphingobium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.84
-
 substrate: bifenthrin, pH 7.5, 37°C Sphingobium sp.
1.52
-
 substrate: deltamethrin, pH 7.5, 37°C Sphingobium sp.
1.75
-
 substrate: fenvalerate, pH 7.5, 37°C Sphingobium sp.
2.46
-
 substrate: cyhalothrin, pH 7.5, 37°C Sphingobium sp.
4.86
-
 substrate: trans-cypermethrin, pH 7.5, 37°C Sphingobium sp.
4.95
-
 substrate: cis-cypermethrin, pH 7.5, 37°C Sphingobium sp.
5.02
-
 substrate: fenpropathrin, pH 7.5, 37°C Sphingobium sp.
5.76
-
 substrate: cis-permethrin, pH 7.5, 37°C Sphingobium sp.
5.82
-
 substrate: trans-permethrin, pH 7.5, 37°C Sphingobium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(alphaR)-fenpropathrin + H2O both stereoisomers of fenpropathrin: (alphaR)- and (alphaS)-fenpropathrin are hydrolyzed at an appropriately equal rate Sphingobium sp. 3-phenoxybenzaldehyde + 2,2,3,3-tetramethylcyclopropanecarboxylate + cyanide
-
 ?
(alphaR,2R)-fenvalerate + H2O the four stereoisomers of fenvalerate: (alphaS,2R)-fenvalerate, (alphaR,2S)-fenvalerate, (alphaS,2S)-fenvalerate and (alphaR,2R)-fenvalerate are hydrolyzed at an appropriately equal rate Sphingobium sp. 3-phenoxybenzaldehyde + (2R)-2-(4-chlorophenyl)-3-methylbutanoate + cyanide
-
 ?
(alphaR,2S)-fenvalerate + H2O the four stereoisomers of fenvalerate: (alphaS,2R)-fenvalerate, (alphaR,2S)-fenvalerate, (alphaS,2S)-fenvalerate and (alphaR,2R)-fenvalerate are hydrolyzed at an appropriately equal rate Sphingobium sp. 3-phenoxybenzaldehyde + (2S)-2-(4-chlorophenyl)-3-methylbutanoate + cyanide
-
 ?
(alphaS)-fenpropathrin + H2O both stereoisomers of fenpropathrin: (alphaR)- and (alphaS)-fenpropathrin are hydrolyzed at an appropriately equal rate Sphingobium sp. 3-phenoxybenzaldehyde + 2,2,3,3-tetramethylcyclopropanecarboxylate + cyanide
-
 ?
(alphaS,2R)-fenvalerate + H2O the four stereoisomers of fenvalerate: (alphaS,2R)-fenvalerate, (alphaR,2S)-fenvalerate, (alphaS,2S)-fenvalerate and (alphaR,2R)-fenvalerate are hydrolyzed at an appropriately equal rate Sphingobium sp. 3-phenoxybenzaldehyde + (2R)-2-(4-chlorophenyl)-3-methylbutanoate + cyanide
-
 ?
(alphaS,2S)-fenvalerate + H2O the four stereoisomers of fenvalerate: (alphaS,2R)-fenvalerate, (alphaR,2S)-fenvalerate, (alphaS,2S)-fenvalerate and (alphaR,2R)-fenvalerate are hydrolyzed at an appropriately equal rate Sphingobium sp. 3-phenoxybenzaldehyde + (2S)-2-(4-chlorophenyl)-3-methylbutanoate + cyanide
-
 ?
bifenthrin + H2O
-
 Sphingobium sp. (2-methylbiphenyl-3-yl)methanol + 3-[(1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl]-2,2-dimethylcyclopropanecarboxylate
-
 ?
cis-cypermethrin + H2O
-
 Sphingobium sp. 3-phenoxybenzaldehyde + (1S,3S)-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide
-
 ?
cis-permethrin + H2O
-
 Sphingobium sp. (3-phenoxyphenyl)methanol + (1S,3S)-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate
-
 ?
cyhalothrin + H2O
-
 Sphingobium sp. 3-phenoxybenzaldehyde + 3-[(1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl]-2,2-dimethylcyclopropanecarboxylate + cyanide
-
 ?
deltamethrin + H2O
-
 Sphingobium sp. 3-phenoxybenzaldehyde + (1R,3R)-3-(2,2-dibromoethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide
-
 ?
additional information the enzyme is involved in degradation of pyrethroids Sphingobium sp. ?
-
 ?
trans-cypermethrin + H2O
-
 Sphingobium sp. 3-phenoxybenzaldehyde + (1S,3R)-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide
-
 ?
trans-permethrin + H2O
-
 Sphingobium sp. (3-phenoxyphenyl)methanol + (1S,3R)-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 31000, SDS-PAGE Sphingobium sp.
monomer 1 * 30835, calculated from sequence Sphingobium sp.

Synonyms

Synonyms Comment Organism
pyrethroid-hydrolyzing carboxylesterase
-
 Sphingobium sp.
pytH
-
 Sphingobium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Sphingobium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 1 h, stable up to Sphingobium sp.
60
-
 1 h, 45% loss of activity Sphingobium sp.
70
-
 1 h, complete inactivation Sphingobium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.44
-
 bifenthrin pH 7.5, 37°C Sphingobium sp.
0.79
-
 deltamethrin pH 7.5, 37°C Sphingobium sp.
0.91
-
 (alphaS,2R)-fenvalerate pH 7.5, 37°C Sphingobium sp.
0.91
-
 (alphaS,2S)-fenvalerate pH 7.5, 37°C Sphingobium sp.
0.91
-
 (alphaR,2R)-fenvalerate pH 7.5, 37°C Sphingobium sp.
0.91
-
 (alphaR,2S)-fenvalerate pH 7.5, 37°C Sphingobium sp.
1.28
-
 cyhalothrin pH 7.5, 37°C Sphingobium sp.
2.53
-
 trans-cypermethrin pH 7.5, 37°C Sphingobium sp.
2.57
-
 cis-cypermethrin pH 7.5, 37°C Sphingobium sp.
2.61
-
 (alphaR)-fenpropathrin pH 7.5, 37°C Sphingobium sp.
2.61
-
 (alphaS)-fenpropathrin pH 7.5, 37°C Sphingobium sp.
3
-
 cis-permethrin pH 7.5, 37°C Sphingobium sp.
3.03
-
 trans-permethrin pH 7.5, 37°C Sphingobium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Sphingobium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9 4 h, enzyme retains more than 80% of its activity in this range Sphingobium sp.

Cofactor

Cofactor Comment Organism Structure
additional information no cofactors were required for enzyme activity Sphingobium sp.

pI Value

Organism Comment pI Value Maximum pI Value
Sphingobium sp. isoelectric focusing, pH-range: 3.5-10.0
-
 4.85

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
150
-
 4-nitrophenyl hexanoate pH 7.5, 37°C Sphingobium sp.
270
-
 bifenthrin pH 7.5, 37°C Sphingobium sp.
670
-
 4-nitrophenyl butanoate pH 7.5, 37°C Sphingobium sp.
1000
-
 deltamethrin pH 7.5, 37°C Sphingobium sp.
1370
-
 4-nitrophenyl acetate pH 7.5, 37°C Sphingobium sp.
1560
-
 (alphaS,2R)-fenvalerate pH 7.5, 37°C Sphingobium sp.
1560
-
 (alphaS,2S)-fenvalerate pH 7.5, 37°C Sphingobium sp.
1560
-
 (alphaR,2R)-fenvalerate pH 7.5, 37°C Sphingobium sp.
1560
-
 (alphaR,2S)-fenvalerate pH 7.5, 37°C Sphingobium sp.
3670
-
 cyhalothrin pH 7.5, 37°C Sphingobium sp.
23010
-
 trans-cypermethrin pH 7.5, 37°C Sphingobium sp.
23870
-
 cis-cypermethrin pH 7.5, 37°C Sphingobium sp.
24670
-
 (alphaR)-fenpropathrin pH 7.5, 37°C Sphingobium sp.
24670
-
 (alphaS)-fenpropathrin pH 7.5, 37°C Sphingobium sp.
46150
-
 cis-permethrin pH 7.5, 37°C Sphingobium sp.
48900
-
 trans-permethrin pH 7.5, 37°C Sphingobium sp.