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Literature summary for 3.1.1.84 extracted from
- Identification of a cocaine esterase in a strain of Pseudomonas maltophilia (1992), J. Bacteriol., 174, 2087-2094.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition by 1 mM eserine or 1 mM p-hydroxymercuribenzoate. Cocaine esterase shows product inhibition with neither benzoate nor ecgonine methyl ester | Stenotrophomonas maltophilia | |
| phenylmethylsulfonyl fluoride | 1 mM, complete inhibition | Stenotrophomonas maltophilia |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.36 | - |
cocaine | pH 7.0, 30°C | Stenotrophomonas maltophilia | |
| 1.75 | - |
ethyl 2-hydroxybenzoate | pH 7.0, 30°C | Stenotrophomonas maltophilia | |
| 1.89 | - |
ethyl benzoate | pH 7.0, 30°C | Stenotrophomonas maltophilia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
in the presence of the solubilizing agent cholate, gel filtration | Stenotrophomonas maltophilia |
| 129000 | - |
1 * 129000, SDS-PAGE | Stenotrophomonas maltophilia |
| 410000 | - |
in the absence of cholate, cocaine esterase has a native MW of 410000 and probably existed as a tetramer, gel filtration | Stenotrophomonas maltophilia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cocaine + H2O | Stenotrophomonas maltophilia | strain MB11L of Pseudomonas maltophilia is capable of using cocaine as its sole carbon and energy source | ecgonine methyl ester + benzoate | - |
? | |
| cocaine + H2O | Stenotrophomonas maltophilia MB11L | strain MB11L of Pseudomonas maltophilia is capable of using cocaine as its sole carbon and energy source | ecgonine methyl ester + benzoate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Stenotrophomonas maltophilia | - |
- |
- |
| Stenotrophomonas maltophilia MB11L | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Stenotrophomonas maltophilia |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
- |
Stenotrophomonas maltophilia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cocaine + H2O | - |
Stenotrophomonas maltophilia | ecgonine methyl ester + benzoate | - |
? | |
| cocaine + H2O | strain MB11L of Pseudomonas maltophilia is capable of using cocaine as its sole carbon and energy source | Stenotrophomonas maltophilia | ecgonine methyl ester + benzoate | - |
? | |
| cocaine + H2O | - |
Stenotrophomonas maltophilia MB11L | ecgonine methyl ester + benzoate | - |
? | |
| cocaine + H2O | strain MB11L of Pseudomonas maltophilia is capable of using cocaine as its sole carbon and energy source | Stenotrophomonas maltophilia MB11L | ecgonine methyl ester + benzoate | - |
? | |
| ethyl 2-hydroxybenzoate + H2O | - |
Stenotrophomonas maltophilia | ethanol + 2-hydroxybenzoate | - |
? | |
| ethyl 2-hydroxybenzoate + H2O | - |
Stenotrophomonas maltophilia MB11L | ethanol + 2-hydroxybenzoate | - |
? | |
| ethyl benzoate + H2O | - |
Stenotrophomonas maltophilia | ethanol + benzoate | - |
? | |
| ethyl benzoate + H2O | - |
Stenotrophomonas maltophilia MB11L | ethanol + benzoate | - |
? | |
| additional information | no activity with: atropine, ethyl 3-hydroxybenzoate or ethyl 4-hydroxybenzoate | Stenotrophomonas maltophilia | ? | - |
? | |
| additional information | no activity with: atropine, ethyl 3-hydroxybenzoate or ethyl 4-hydroxybenzoate | Stenotrophomonas maltophilia MB11L | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 129000, SDS-PAGE | Stenotrophomonas maltophilia |
| tetramer | in the absence of cholate, cocaine esterase has a native MW of 410000 and probably existed as a tetramer | Stenotrophomonas maltophilia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Stenotrophomonas maltophilia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Stenotrophomonas maltophilia |
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