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Literature summary for 3.1.1.82 extracted from

  • Christ, B.; Schelbert, S.; Aubry, S.; Süssenbacher, I.; Müller, T.; Kräutler, B.; Hörtensteiner, S.
    MES16, a member of the methylesterase protein family, specifically demethylates fluorescent chlorophyll catabolites during chlorophyll breakdown in Arabidopsis (2012), Plant Physiol., 158, 628-641.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Arabidopsis thaliana 5829
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information recombinant MES16 protein is able to demethylate both pheophorbide a and primary fluorescent chlorophyll catabolites (pFCC), but in vivo, MES16 specifically acts on FCCs Arabidopsis thaliana ?
-
 ?
pheophorbide a + H2O pheophorbide a is not an in vivo substrate for MES16 Arabidopsis thaliana pyropheophorbide a + methanol + CO2
-
 ?

Synonyms

Synonyms Comment Organism
MES16
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction MES16-deficient mutants (mes16) are still able to degrade chlorophyll, but they accumulate FCCs (fluorescent chlorophyll) and NCCs (nonfluorescent chlorophyll) catabolites with an intact C132-carboxymethyl group. As a consequence, FCC-to-NCC isomerization is compromised and the mutants accumulate large quantities of FCCs, which causes senescent leaves to fluoresce under UV light Arabidopsis thaliana