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Literature summary for 3.1.1.82 extracted from
- Expression and purification of pheophorbidase, an enzyme catalyzing the formation of pyropheophorbide during chlorophyll degradation: comparison with the native enzyme (2008), Photochem. Photobiol. Sci., 7, 1260-1266.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant PPD is produced using a glutathione S-transferase (GST) fusion system. The PPD and GST genes are fused to a pGEX-2T vector and expressed in Escherichia coli BL21(DE3)pLysS strain under the control of a T7 promoter as a fusion protein | Raphanus sativus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.955 | - |
pheophorbide a | PPD-GST has about 6fold lower affinity for Pheid a than native PPDs | Raphanus sativus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
PPD-GST fusion protein, SDS-PAGE | Raphanus sativus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pheophorbide a + H2O | Raphanus sativus | - |
pyropheophorbide a + methanol + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Raphanus sativus | Q2V0W1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| single-step affinity chromatography through a GSTrap FF column | Raphanus sativus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Raphanus sativus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pheophorbide a + H2O | - |
Raphanus sativus | pyropheophorbide a + methanol + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pheophorbidase | - |
Raphanus sativus |
| Ppd | - |
Raphanus sativus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 80 | - |
Raphanus sativus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
50% loss of activity | Raphanus sativus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | 60 mM MES-HEPES-tricine buffer is used as an incubation medium in the pH range of 5.5 to 9.0 | Raphanus sativus |
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