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Literature summary for 3.1.1.82 extracted from

  • Suzuki, Y.; Amano, T.; Shioi, Y.
    Characterization and cloning of the chlorophyll-degrading enzyme pheophorbidase from cotyledons of radish (2006), Plant Physiol., 140, 716-725.
    View publication on PubMed
Search for more literature for 3.1.1.82

Cloned(Commentary)

Cloned (Comment) Organism
PPD type 1 Raphanus sativus
PPD type 2 Raphanus sativus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0141
-
 pheophorbide a PPD type 1 Raphanus sativus
0.0151
-
 pheophorbide a PPD type 2 Raphanus sativus
0.037
-
 bacteriopheophorbide a PPD type 2 Raphanus sativus
0.039
-
 bacteriopheophorbide a PPD type 1 Raphanus sativus
0.232
-
 pheophorbide b PPD type 2 Raphanus sativus
0.243
-
 pheophorbide b PPD type 1 Raphanus sativus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
113000
-
 PPD type 1, gel filtration Raphanus sativus
113000
-
 PPD type 2, gel filtration Raphanus sativus

Organism

Organism UniProt Comment Textmining
Raphanus sativus Q2V0W1
-
-

Purification (Commentary)

Purification (Comment) Organism
PPD type 1 Raphanus sativus
PPD type 2 Raphanus sativus

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon PPD type 1 Raphanus sativus
-
cotyledon PPD type 2 Raphanus sativus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0137
-
-
 Raphanus sativus
0.021
-
-
 Raphanus sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bacteriopheophorbide a + H2O PPD type 1 Raphanus sativus ?
-
 ?
bacteriopheophorbide a + H2O PPD type 2 Raphanus sativus ?
-
 ?
additional information the enzyme is extremely specific for pheophorbides of the dihydroporphyrin and tetrahydroporphyrin type Raphanus sativus ?
-
 ?
pheophorbide a + H2O PPD type 1 Raphanus sativus pyropheophorbide a + methanol + CO2
-
 ?
pheophorbide a + H2O PPD type 2 Raphanus sativus pyropheophorbide a + methanol + CO2
-
 ?
pheophorbide b + H2O PPD type 1 Raphanus sativus ?
-
 ?
pheophorbide b + H2O PPD type 2 Raphanus sativus ?
-
 ?

Synonyms

Synonyms Comment Organism
PPD type 1
-
 Raphanus sativus
PPD type 2
-
 Raphanus sativus