Activating Compound | Comment | Organism | Structure |
---|---|---|---|
protease K | activity is enhanced up to 109% after preincubations with trypsin and protease K at 37°C for 30 and 60 min | Kurthia huakuii | |
Trypsin | activity is enhanced up to 109% after preincubations with trypsin and protease K at 37°C for 30 and 60 min | Kurthia huakuii |
Application | Comment | Organism |
---|---|---|
drug development | the enzyme significantly inhibits the biofilm formation and attenuates extracellular proteolytic activity and pyocyanin production of Pseudomonas aeruginosa PAO1, which indicates the potential application of AiiK as a biocontrol agent or an anti-pathogenic drug | Kurthia huakuii |
pharmacology | the enzyme significantly inhibits the biofilm formation and attenuates extracellular proteolytic activity and pyocyanin production of Pseudomonas aeruginosa PAO1, which indicates the potential application of AiiK as a biocontrol agent or an anti-pathogenic drug | Kurthia huakuii |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) | Kurthia huakuii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-chymotrypsin | the enzyme maintains 87% and 101% activity after preincubations with alpha-chymotrypsin at 37°C for 30 min and 60 min | Kurthia huakuii | |
Ca2+ | 1 mM, 30% inhibition. 5 mM, 45% inhibition | Kurthia huakuii | |
Co2+ | 5 mM, 50% inhibition | Kurthia huakuii | |
Cu2+ | 1 mM, complete inhibition | Kurthia huakuii | |
EDTA | 5 mM, 50% inhibition | Kurthia huakuii | |
Fe2+ | 5 mM, 25% inhibition | Kurthia huakuii | |
Mg2+ | 5 mM, 10% loss of activity | Kurthia huakuii | |
Ni2+ | 5 mM, 50% inhibition | Kurthia huakuii | |
Zn2+ | 1 mM, 20% inhibition. 5 mM, 90% inhibition | Kurthia huakuii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | - |
N-tetradecanoyl-L-homoserine lactone | pH 7.4, 25°C | Kurthia huakuii | |
0.066 | - |
N-3-oxohexanoyl-L-homoserine lactone | pH 7.4, 25°C | Kurthia huakuii | |
0.204 | - |
N-hexanoyl-L-homoserine lactone | pH 7.4, 25°C | Kurthia huakuii | |
0.315 | - |
N-decanoyl-L-homoserine lactone | pH 7.4, 25°C | Kurthia huakuii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 1.25fold activation | Kurthia huakuii | |
Mn2+ | 1 mM, 1.4fold activation | Kurthia huakuii | |
Ni2+ | 1 mM, 1.5fold activation | Kurthia huakuii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE, purified fusion protein containing complex soluble tags (Trx-Tag, His-Tag and S-Tag) | Kurthia huakuii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kurthia huakuii | - |
- |
- |
Kurthia huakuii LAM0618T | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Kurthia huakuii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-3-oxohexanoyl-L-homoserine lactone + H2O | - |
Kurthia huakuii | N-3-oxohexanoyl-L-homoserine | - |
? | |
N-3-oxohexanoyl-L-homoserine lactone + H2O | - |
Kurthia huakuii LAM0618T | N-3-oxohexanoyl-L-homoserine | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | - |
Kurthia huakuii | N-decanoyl-L-homoserine | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | - |
Kurthia huakuii LAM0618T | N-decanoyl-L-homoserine | - |
? | |
N-hexanoyl-L-homoserine lactone + H2O | - |
Kurthia huakuii | N-hexanoyl-L-homoserine | - |
? | |
N-hexanoyl-L-homoserine lactone + H2O | - |
Kurthia huakuii LAM0618T | N-hexanoyl-L-homoserine | - |
? | |
N-tetradecanoyl-L-homoserine lactone + H2O | - |
Kurthia huakuii | N-tetradecanoyl-L-homoserine | - |
? | |
N-tetradecanoyl-L-homoserine lactone + H2O | - |
Kurthia huakuii LAM0618T | N-tetradecanoyl-L-homoserine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31760, calculated from sequence | Kurthia huakuii |
Synonyms | Comment | Organism |
---|---|---|
AHL lactonase | - |
Kurthia huakuii |
AiiK | - |
Kurthia huakuii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Kurthia huakuii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 65 | 5°C: about 40% of maximal activity, 65°C: about 35% of maximal activity | Kurthia huakuii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
about 50% loss of activity after 4 h, about 80% loss of activity after 12 h | Kurthia huakuii |
45 | - |
about 50% loss of activity after 4 h, about 90% loss of activity after 12 h | Kurthia huakuii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
N-tetradecanoyl-L-homoserine lactone | pH 7.4, 25°C | Kurthia huakuii | |
0.168 | - |
N-3-oxohexanoyl-L-homoserine lactone | pH 7.4, 25°C | Kurthia huakuii | |
0.649 | - |
N-hexanoyl-L-homoserine lactone | pH 7.4, 25°C | Kurthia huakuii | |
1.321 | - |
N-decanoyl-L-homoserine lactone | pH 7.4, 25°C | Kurthia huakuii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Kurthia huakuii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | AiiK is stable at pH values ranging from 6 to 8, maintaining over 70% activity after preincubations for 30 min at 25°C | Kurthia huakuii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.92 | - |
N-tetradecanoyl-L-homoserine lactone | pH 7.4, 25°C | Kurthia huakuii | |
2.7 | - |
N-3-oxohexanoyl-L-homoserine lactone | pH 7.4, 25°C | Kurthia huakuii | |
3.2 | - |
N-hexanoyl-L-homoserine lactone | pH 7.4, 25°C | Kurthia huakuii | |
3.9 | - |
N-decanoyl-L-homoserine lactone | pH 7.4, 25°C | Kurthia huakuii |