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Literature summary for 3.1.1.81 extracted from

  • Garge, S.S.; Nerurkar, A.S.
    Attenuation of quorum sensing regulated virulence of Pectobacterium carotovorum subsp. carotovorum through an AHL lactonase produced by Lysinibacillus sp. Gs50 (2016), PLoS ONE, 11, e0167344 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.81

Application

Application Comment Organism
agriculture potential of Lysinibacillus sp. Gs50 as a preventive and curative biocontrol agent. Lysinibacillus sp. Gs50 can degrade acyl homoserine lactones produced by Pectobacterium carotovorum subsp. carotovorum, a common cause of soft rot. This quorum quenching activity causes a decrease in production of plant cell wall degrading enzymes of Pectobacterium carotovorum subsp. carotovorum and attenuates symptoms of soft rot in experimental infection of potato, carrot and cucumber Lysinibacillus sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Lysinibacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1 mM, complete inhibition Lysinibacillus sp.
Cd2+ 1 mM, complete inhibition Lysinibacillus sp.
Cu2+ 1 mM, complete inhibition Lysinibacillus sp.
EDTA 1 mM, complete inhibition Lysinibacillus sp.
Fe2+ 1 mM, partial inhibition Lysinibacillus sp.
Mg2+ 1 mM, partial inhibition Lysinibacillus sp.
Mn2+ 1 mM, complete inhibition Lysinibacillus sp.
Zn2+ 1 mM, partial inhibition Lysinibacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003089
-
 N-hexanoyl-L-homoserine lactone pH 7.4, 30°C Lysinibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 SDS-PAGE Lysinibacillus sp.

Organism

Organism UniProt Comment Textmining
Lysinibacillus sp. A0A142LT01
-
-
Lysinibacillus sp. Gs50 A0A142LT01
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lysinibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-hexanoyl-L-homoserine lactone + H2O
-
 Lysinibacillus sp. N-hexanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O
-
 Lysinibacillus sp. Gs50 N-hexanoyl-L-homoserine
-
 ?

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE Lysinibacillus sp.

Synonyms

Synonyms Comment Organism
adeH
-
 Lysinibacillus sp.
AHL lactonase
-
 Lysinibacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Lysinibacillus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 37 20°C: about 50% of maximal activity, 37°C: about 65% of maximal activity Lysinibacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 2 h, 50% loss of activity Lysinibacillus sp.
30
-
 high thermal stability at Lysinibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Lysinibacillus sp.

pH Range

pH Minimum pH Maximum Comment Organism
4.8 8.3 pH 4.8: about 45% of maximal activity, pH 8.3: about 50% of maximal activity Lysinibacillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 unstable at pH 9.0 and below pH 5.0 Lysinibacillus sp.