Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus thuringiensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
N-hexanoyl-L-homoserine lactone | Mn2+-form of AHL lactonase, at pH 7.4 and at 28°C | Bacillus thuringiensis | |
0.24 | - |
N-hexanoyl-L-homoserine lactone | Cd2+-form of AHL lactonase, at pH 7.4 and at 28°C | Bacillus thuringiensis | |
0.36 | - |
N-hexanoyl-L-homoserine lactone | Co2+-form of AHL lactonase, at pH 7.4 and at 28°C | Bacillus thuringiensis | |
5.6 | - |
N-hexanoyl-L-homoserine lactone | Zn2+-form of AHL lactonase, at pH 7.4 and at 28°C | Bacillus thuringiensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thuringiensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
amylose-agarose resin column chromatography and DEAE-Sepharose column chromatography | Bacillus thuringiensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-(3-oxooctanoyl)-L-homoserine lactone + H2O | - |
Bacillus thuringiensis | N-(3-oxooctanoyl)-L-homoserine | - |
? | |
N-hexanoyl-L-homoserine lactone + H2O | - |
Bacillus thuringiensis | N-hexanoyl-L-homoserine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AHL lactonase | - |
Bacillus thuringiensis |
AiiA | - |
Bacillus thuringiensis |
N-acyl-L-homoserine lactone lactonase | - |
Bacillus thuringiensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
91 | - |
N-hexanoyl-L-homoserine lactone | Zn2+-form of AHL lactonase, at pH 7.4 and at 28°C | Bacillus thuringiensis | |
330 | - |
N-hexanoyl-L-homoserine lactone | Mn2+-form of AHL lactonase, at pH 7.4 and at 28°C | Bacillus thuringiensis | |
480 | - |
N-hexanoyl-L-homoserine lactone | Cd2+-form of AHL lactonase, at pH 7.4 and at 28°C | Bacillus thuringiensis | |
510 | - |
N-hexanoyl-L-homoserine lactone | Co2+-form of AHL lactonase, at pH 7.4 and at 28°C | Bacillus thuringiensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16 | - |
N-hexanoyl-L-homoserine lactone | Zn2+-form of AHL lactonase, at pH 7.4 and at 28°C | Bacillus thuringiensis | |
1400 | - |
N-hexanoyl-L-homoserine lactone | Co2+-form of AHL lactonase, at pH 7.4 and at 28°C | Bacillus thuringiensis | |
1800 | - |
N-hexanoyl-L-homoserine lactone | Mn2+-form of AHL lactonase, at pH 7.4 and at 28°C | Bacillus thuringiensis | |
2000 | - |
N-hexanoyl-L-homoserine lactone | Cd2+-form of AHL lactonase, at pH 7.4 and at 28°C | Bacillus thuringiensis |