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Literature summary for 3.1.1.81 extracted from

  • Seo, M.J.; Lee, B.S.; Pyun, Y.R.; Park, H.
    Isolation and characterization of N-acylhomoserine lactonase from the Thermophilic bacterium, Geobacillus caldoxylosilyticus YS-8 (2011), Biosci. Biotechnol. Biochem., 75, 1789-1795.
    View publication on PubMed
Search for more literature for 3.1.1.81

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 x * 32000, SDS-PAGE Parageobacillus caldoxylosilyticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Parageobacillus caldoxylosilyticus the AHL-degrading enzyme catalyzes the lactone ring opening of N-3-oxohexanoyl-L-homoserine lactone and N-hexanoyl-L-homoserine lactone by hydrolyzing the lactones ?
-
 ?
additional information Parageobacillus caldoxylosilyticus YS-8 the AHL-degrading enzyme catalyzes the lactone ring opening of N-3-oxohexanoyl-L-homoserine lactone and N-hexanoyl-L-homoserine lactone by hydrolyzing the lactones ?
-
 ?
N-3-oxo-dodecanoyl-L-homoserine lactone + H2O Parageobacillus caldoxylosilyticus
-
 N-3-oxo-dodecanoyl-L-homoserine
-
 ?
N-3-oxo-hexanoyl-L-homoserine lactone + H2O Parageobacillus caldoxylosilyticus lower activity N-3-oxo-hexanoyl-L-homoserine
-
 ?
N-decanoyl-L-homoserine lactone + H2O Parageobacillus caldoxylosilyticus
-
 N-decanoyl-L-homoserine
-
 ?
N-decanoyl-L-homoserine lactone + H2O Parageobacillus caldoxylosilyticus YS-8
-
 N-decanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O Parageobacillus caldoxylosilyticus lower activity N-hexanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O Parageobacillus caldoxylosilyticus YS-8 lower activity N-hexanoyl-L-homoserine
-
 ?
N-octanoyl-L-homoserine lactone + H2O Parageobacillus caldoxylosilyticus lower activity N-octanoyl-L-homoserine
-
 ?
N-octanoyl-L-homoserine lactone + H2O Parageobacillus caldoxylosilyticus YS-8 lower activity N-octanoyl-L-homoserine
-
 ?

Organism

Organism UniProt Comment Textmining
Parageobacillus caldoxylosilyticus
-
-
-
Parageobacillus caldoxylosilyticus YS-8
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 201.5fold by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, gel filtration, and another step of anion exchange chromatography Parageobacillus caldoxylosilyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
256
-
 purified enzyme, substrate N-3-oxo-dodecanoyl-L-homoserine lactone, pH 6.5, 50°C Parageobacillus caldoxylosilyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity, overview Parageobacillus caldoxylosilyticus ?
-
 ?
additional information the AHL-degrading enzyme catalyzes the lactone ring opening of N-3-oxohexanoyl-L-homoserine lactone and N-hexanoyl-L-homoserine lactone by hydrolyzing the lactones Parageobacillus caldoxylosilyticus ?
-
 ?
additional information substrate specificity, overview Parageobacillus caldoxylosilyticus YS-8 ?
-
 ?
additional information the AHL-degrading enzyme catalyzes the lactone ring opening of N-3-oxohexanoyl-L-homoserine lactone and N-hexanoyl-L-homoserine lactone by hydrolyzing the lactones Parageobacillus caldoxylosilyticus YS-8 ?
-
 ?
N-3-oxo-dodecanoyl-L-homoserine lactone + H2O
-
 Parageobacillus caldoxylosilyticus N-3-oxo-dodecanoyl-L-homoserine
-
 ?
N-3-oxo-hexanoyl-L-homoserine lactone + H2O lower activity Parageobacillus caldoxylosilyticus N-3-oxo-hexanoyl-L-homoserine
-
 ?
N-decanoyl-L-homoserine lactone + H2O
-
 Parageobacillus caldoxylosilyticus N-decanoyl-L-homoserine
-
 ?
N-decanoyl-L-homoserine lactone + H2O
-
 Parageobacillus caldoxylosilyticus YS-8 N-decanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O lower activity Parageobacillus caldoxylosilyticus N-hexanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O lower activity Parageobacillus caldoxylosilyticus YS-8 N-hexanoyl-L-homoserine
-
 ?
N-octanoyl-L-homoserine lactone + H2O lower activity Parageobacillus caldoxylosilyticus N-octanoyl-L-homoserine
-
 ?
N-octanoyl-L-homoserine lactone + H2O lower activity Parageobacillus caldoxylosilyticus YS-8 N-octanoyl-L-homoserine
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Parageobacillus caldoxylosilyticus

Synonyms

Synonyms Comment Organism
AHL lactonase
-
 Parageobacillus caldoxylosilyticus
AHL-degrading enzyme
-
 Parageobacillus caldoxylosilyticus
N-acylhomoserine lactonase
-
 Parageobacillus caldoxylosilyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Parageobacillus caldoxylosilyticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 activity range, 30% of maximal activity at 80°C, profile, overview Parageobacillus caldoxylosilyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 purified enzyme, 3 h, 100% of maximal activity remaining Parageobacillus caldoxylosilyticus
50
-
 purified enzyme, 3 h, 80% of maximal activity remaining Parageobacillus caldoxylosilyticus
60
-
 purified enzyme, 3 h, 50% of maximal activity remaining Parageobacillus caldoxylosilyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Parageobacillus caldoxylosilyticus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8 activity range, profile, overview Parageobacillus caldoxylosilyticus

General Information

General Information Comment Organism
physiological function the enzyme is involved in quorum sensing, a regulatory mechanism of cell-to-cell communication in response to environmental conditions Parageobacillus caldoxylosilyticus