Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain DH5alpha | Bacillus thuringiensis serovar kurstaki |
gene aiiA, expression in Escherichia coli strain DH5alpha fused to the maltose binding protein with the TEV protease cleavage site ENLYFQ*G | Bacillus thuringiensis serovar kurstaki |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, different enzyme constitutions | Bacillus thuringiensis serovar kurstaki | |
6.7 | - |
N-hexanoyl-(S)-homoserine lactone | pH 7.4, 28°C, purified detagged recombinant enzyme | Bacillus thuringiensis serovar kurstaki |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | dinuclear form, 2 Zn2+ ions per enzyme molecule, preparation of the apoenzyme, which is inactive, and reconstitution with Zn2+ at pH 7.1 restoring about 60% of native enzyme activity, metal content determination, overview | Bacillus thuringiensis serovar kurstaki | |
Zn2+ | the enzyme is a metalloprotein | Bacillus thuringiensis serovar kurstaki |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, SDS-PAGE | Bacillus thuringiensis serovar kurstaki |
29000 | - |
x * 29000, recombinant detagged enzyme, SDS-PAGE | Bacillus thuringiensis serovar kurstaki |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bacillus thuringiensis serovar kurstaki | N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence | ? | - |
? | |
additional information | Bacillus thuringiensis serovar kurstaki 4A3 | N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence | ? | - |
? | |
additional information | Bacillus thuringiensis serovar kurstaki BGSC 4A3 | N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thuringiensis serovar kurstaki | P0CJ63 | - |
- |
Bacillus thuringiensis serovar kurstaki | P0CJ63 | gene aiiA | - |
Bacillus thuringiensis serovar kurstaki 4A3 | P0CJ63 | - |
- |
Bacillus thuringiensis serovar kurstaki BGSC 4A3 | P0CJ63 | gene aiiA | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus thuringiensis serovar kurstaki |
recombinant maltose binding protein-fusion enzyme from Escherichia coli strain DH5alpha to over 95% purity, the fusion tag is cleaved off by TEV protease, the detagged enzyme is purified by DEAE ion exchange chromatography, and amylose affinity chromatography to remove traces of MBP, further purification by hydrophobic interaction chromatography in presence of 1.6-2.0 M ammonium sulfate, and dialysis | Bacillus thuringiensis serovar kurstaki |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bacillus thuringiensis serovar kurstaki |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence | Bacillus thuringiensis serovar kurstaki | ? | - |
? | |
additional information | N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence | Bacillus thuringiensis serovar kurstaki 4A3 | ? | - |
? | |
additional information | N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence | Bacillus thuringiensis serovar kurstaki BGSC 4A3 | ? | - |
? | |
N-hexanoyl-(S)-homoserine lactone | - |
Bacillus thuringiensis serovar kurstaki | N-hexanoyl-(S)-homoserine | - |
? | |
N-hexanoyl-(S)-homoserine lactone | - |
Bacillus thuringiensis serovar kurstaki 4A3 | N-hexanoyl-(S)-homoserine | - |
? | |
N-hexanoyl-(S)-homoserine lactone | - |
Bacillus thuringiensis serovar kurstaki BGSC 4A3 | N-hexanoyl-(S)-homoserine | - |
? | |
N-hexanoyl-(S)-homoserine lactone + H2O | no activity with N-hexanoyl-(R)-homoserine lactone | Bacillus thuringiensis serovar kurstaki | N-hexanoyl-(S)-homoserine | - |
? | |
N-hexanoyl-(S)-homoserine lactone + H2O | no activity with N-hexanoyl-(R)-homoserine lactone | Bacillus thuringiensis serovar kurstaki 4A3 | N-hexanoyl-(S)-homoserine | - |
? | |
N-hexanoyl-(S)-homoserine lactone + H2O | no activity with N-hexanoyl-(R)-homoserine lactone | Bacillus thuringiensis serovar kurstaki BGSC 4A3 | N-hexanoyl-(S)-homoserine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, SDS-PAGE | Bacillus thuringiensis serovar kurstaki |
? | x * 29000, recombinant detagged enzyme, SDS-PAGE | Bacillus thuringiensis serovar kurstaki |
Synonyms | Comment | Organism |
---|---|---|
AHL lactonase | - |
Bacillus thuringiensis serovar kurstaki |
quorum-quenching lactonase | - |
Bacillus thuringiensis serovar kurstaki |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Bacillus thuringiensis serovar kurstaki |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
117 | - |
N-hexanoyl-(S)-homoserine lactone | pH 7.4, 28°C, purified detagged recombinant enzyme | Bacillus thuringiensis serovar kurstaki |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bacillus thuringiensis serovar kurstaki |