Cloned (Comment) | Organism |
---|---|
gene aiiA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH5alpha | Bacillus cereus |
gene aiiA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH5alpha | Bacillus mycoides |
gene aiiA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH5alpha | Bacillus sp. (in: Bacteria) |
gene aiiA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH5alpha | Bacillus thuringiensis |
Protein Variants | Comment | Organism |
---|---|---|
D191S | site-directed mutagenesis, mutation of a zinc-binding motif residue, inactive mutant | Bacillus sp. (in: Bacteria) |
D236S | site-directed mutagenesis, the mutation does not affect the enzyme activity | Bacillus sp. (in: Bacteria) |
H235S | site-directed mutagenesis, the mutation does not affect the enzyme activity | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | metallohydrolase containing the conserved zinc-binding motif | Bacillus cereus | |
Zn2+ | metallohydrolase containing the conserved zinc-binding motif | Bacillus mycoides | |
Zn2+ | metallohydrolase containing the conserved zinc-binding motif | Bacillus thuringiensis | |
Zn2+ | metallohydrolase containing the conserved zinc-binding motif, residues H106, H109, D108, and H169 of the strain 240B1 enzyme are required for Zn2+ binding and full enzyme activity, overview | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acyl-(S)-homoserine lactone + H2O | Bacillus cereus | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants | N-acyl-(S)-homoserine | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | Bacillus mycoides | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants | N-acyl-(S)-homoserine | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | Bacillus sp. (in: Bacteria) | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants | N-acyl-(S)-homoserine | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | Bacillus thuringiensis | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants | N-acyl-(S)-homoserine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Bacillus mycoides | - |
- |
- |
Bacillus sp. (in: Bacteria) | Q8RPW9 | gene aiiA from Bacillus sp. strain COT1; strains 240B1 and COT1 | - |
Bacillus thuringiensis | A3FJ64 | aiiA from strain B1; several subspecies and strains, overview | - |
Bacillus thuringiensis | A3FJ64 | aiiA from strain B2; several subspecies and strains, overview | - |
Bacillus thuringiensis | Q8RJA0 | aiiA from strain B20; several subspecies and strains, overview | - |
Bacillus thuringiensis | Q8RPW5 | aiiA from strain B18; several subspecies and strains, overview | - |
Bacillus thuringiensis | Q8RPW6 | aiiA from strain B17; several subspecies and strains, overview | - |
no activity in Bacillus fusiformis | - |
- |
- |
no activity in Bacillus sphaericus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acyl-(S)-homoserine lactone + H2O | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants | Bacillus cereus | N-acyl-(S)-homoserine | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants | Bacillus mycoides | N-acyl-(S)-homoserine | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants | Bacillus sp. (in: Bacteria) | N-acyl-(S)-homoserine | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants | Bacillus thuringiensis | N-acyl-(S)-homoserine | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | the acylhomoserine lactones are inducers of quorum-sensing signaling | Bacillus cereus | N-acyl-(S)-homoserine | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | the acylhomoserine lactones are inducers of quorum-sensing signaling | Bacillus mycoides | N-acyl-(S)-homoserine | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | the acylhomoserine lactones are inducers of quorum-sensing signaling | Bacillus sp. (in: Bacteria) | N-acyl-(S)-homoserine | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | the acylhomoserine lactones are inducers of quorum-sensing signaling | Bacillus thuringiensis | N-acyl-(S)-homoserine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AHL lactonase | - |
Bacillus cereus |
AHL lactonase | - |
Bacillus mycoides |
AHL lactonase | - |
Bacillus sp. (in: Bacteria) |
AHL lactonase | - |
Bacillus thuringiensis |
AHL-inactivating enzyme | - |
Bacillus cereus |
AHL-inactivating enzyme | - |
Bacillus mycoides |
AHL-inactivating enzyme | - |
Bacillus sp. (in: Bacteria) |
AHL-inactivating enzyme | - |
Bacillus thuringiensis |
AHL-lactonase | - |
Bacillus sp. (in: Bacteria) |
AHL-lactonase | - |
Bacillus thuringiensis |
quorum-quenching N-acyl homoserine lactonase | - |
Bacillus cereus |
quorum-quenching N-acyl homoserine lactonase | - |
Bacillus mycoides |
quorum-quenching N-acyl homoserine lactonase | - |
Bacillus sp. (in: Bacteria) |
quorum-quenching N-acyl homoserine lactonase | - |
Bacillus thuringiensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
in vivo assay at | Bacillus cereus |
28 | - |
in vivo assay at | Bacillus mycoides |
28 | - |
in vivo assay at | Bacillus sp. (in: Bacteria) |
28 | - |
in vivo assay at | Bacillus thuringiensis |