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Literature summary for 3.1.1.8 extracted from

  • Riov, J.; Jaffe, M.J.
    Cholinesterases from plant tissues (1973), Plant Physiol., 51, 520-528.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.8

Inhibitors

Inhibitors Comment Organism Structure
(2-mercaptoethyl)trimethylammonium iodide O,O-diethyl phosphorothioate 0.2 mM, 21% inhibition; i.e. echothiophate Vigna radiata var. radiata
1,5-bis(4-allyldimethylammoniumphenyl)-pentan-3-one 1 mM, 20% inhibition, 10 mM, 65% inhibition; i.e. B.W 284 C51 dibromide Vigna radiata var. radiata
1-methylcarbamoyloxy-naphthalene 0.2 mM, 19% inhibition; i.e. sevin Vigna radiata var. radiata
1-p-menthanol, 2-dimethylamino-, 2,5-dimethylbenzyl chloride Q 80, growth retardant, 0.1 mM, 32% inhibition Vigna radiata var. radiata
ambenonium 1 mM, 18% inhibition, 10 mM 50% inhibition Vigna radiata var. radiata
ammonium(5-hydroxycarvacryl) trimethyl chloride piperidine carboxylate i.e. AMO-1618, growth retardant, 1 mM, 85% inhibition Vigna radiata var. radiata
Diethyl p-nitrophenyl phosphate 0.001 mM, 71% inhibition; i.e. paraoxon Vigna radiata var. radiata
ethopropazine hydrochloride 1 mM, 28% inhibition Vigna radiata var. radiata
neostigmine 0.0006 mM, 50% inhibition Vigna radiata var. radiata
physostigmine 0.42 mM, 50% inhibition; i.e. eserine Vigna radiata var. radiata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.072
-
 Acetylcholine
-
 Vigna radiata var. radiata
0.084
-
 acetylthiocholine
-
 Vigna radiata var. radiata

Organism

Organism UniProt Comment Textmining
Vigna radiata var. radiata
-
 mung bean
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vigna radiata var. radiata

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Vigna radiata var. radiata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.096
-
 acetylcholine Vigna radiata var. radiata

Storage Stability

Storage Stability Organism
4°C, several weeks, no loss of activity, enzyme preparation obtained by direct extraction with (NH4)2SO4 Vigna radiata var. radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylcholine + H2O
-
 Vigna radiata var. radiata acetate + choline
-
 ?
acetylthiocholine + H2O
-
 Vigna radiata var. radiata acetate + thiocholine
-
 ?
butyrylthiocholine + H2O
-
 Vigna radiata var. radiata butanoate + thiocholine
-
 ?
additional information
-
 Vigna radiata var. radiata ?
-
 ?
propionylthiocholine + H2O
-
 Vigna radiata var. radiata propanoate + thiocholine
-
 ?

Synonyms

Synonyms Comment Organism
ChE
-
 Vigna radiata var. radiata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 8.7 acetylcholine Vigna radiata var. radiata

pH Range

pH Minimum pH Maximum Comment Organism
6 10 pH 6.0 and pH 10.0 25% of maximal activity Vigna radiata var. radiata

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0006
-
 neostigmine
-
 Vigna radiata var. radiata
10
-
 ambenonium
-
 Vigna radiata var. radiata