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Literature summary for 3.1.1.8 extracted from

  • Chuiko, G.; Lapkina, L.; Podgornaya, V.
    Substrate-inhibitory analysis of the cholinesterase in the freshwater oligochaete Lumbriculus variegatus (O.F. Mueller, 1773; Lumbriculidae, Oligochaeta) (2011), Biochem. Syst. Ecol., 39, 169-174.
No PubMed abstract available
Search for more literature for 3.1.1.8

Inhibitors

Inhibitors Comment Organism Structure
2,2,-dichlorvinyl dimethyl phosphate irreversible inhibition Lumbriculus variegatus
acetylthiocholine substrate inhibition Lumbriculus variegatus
butyrylthiocholine substrate inhibition Lumbriculus variegatus
additional information not inhibited by propionylthiocholine and acetyl-beta-metylthiocholine Lumbriculus variegatus
paraoxon i.e. diethyl-4-nitrophenylphosphate, irreversible inhibition Lumbriculus variegatus
tetraisopropyl pyrophosphoramide irreversible inhibition Lumbriculus variegatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 propionylthiocholine in 0.1 M phosphate buffer, pH 7.5, at 30°C Lumbriculus variegatus
0.2
-
 butyrylthiocholine in 0.1 M phosphate buffer, pH 7.5, at 30°C Lumbriculus variegatus
0.25
-
 acetyl-beta-methylthiocholine in 0.1 M phosphate buffer, pH 7.5, at 30°C Lumbriculus variegatus
0.27
-
 acetylthiocholine in 0.1 M phosphate buffer, pH 7.5, at 30°C Lumbriculus variegatus

Organism

Organism UniProt Comment Textmining
Lumbriculus variegatus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
 homogenate from frozen worms, using butyrylthiocholine as substrate, in 0.1 M phosphate buffer, pH 7.5, at 30°C Lumbriculus variegatus
0.0109
-
 homogenate from frozen worms, using acetyl-beta-metylthiocholine as substrate, in 0.1 M phosphate buffer, pH 7.5, at 30°C Lumbriculus variegatus
0.0117
-
 homogenate from frozen worms, using propionylthiocholine as substrate, in 0.1 M phosphate buffer, pH 7.5, at 30°C Lumbriculus variegatus
0.0144
-
 homogenate from frozen worms, using acetylthiocholine as substrate, in 0.1 M phosphate buffer, pH 7.5, at 30°C Lumbriculus variegatus
0.0731
-
 homogenate from live worms, using butyrylthiocholine as substrate, in 0.1 M phosphate buffer, pH 7.5, at 30°C Lumbriculus variegatus
0.0736
-
 homogenate from live worms, using acetyl-beta-metylthiocholine as substrate, in 0.1 M phosphate buffer, pH 7.5, at 30°C Lumbriculus variegatus
0.079
-
 homogenate from live worms, using propionylthiocholine as substrate, in 0.1 M phosphate buffer, pH 7.5, at 30°C Lumbriculus variegatus
0.0987
-
 homogenate from live worms, using acetylthiocholine as substrate, in 0.1 M phosphate buffer, pH 7.5, at 30°C Lumbriculus variegatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-beta-methylthiocholine + H2O 75% activity compared to acetylthiocholine Lumbriculus variegatus beta-methylthiocholine + acetate
-
 ?
acetylthiocholine + H2O 100% activity Lumbriculus variegatus thiocholine + acetate
-
 ?
butyrylthiocholine + H2O 74% activity compared to acetylthiocholine Lumbriculus variegatus thiocholine + butyrate
-
 ?
propionylthiocholine + H2O 80% activity compared to acetylthiocholine Lumbriculus variegatus thiocholine + propionate
-
 ?

Synonyms

Synonyms Comment Organism
Acylcholine acylhydrolase
-
 Lumbriculus variegatus
atypical cholinesterase
-
 Lumbriculus variegatus
ChE
-
 Lumbriculus variegatus