Cloned (Comment) | Organism |
---|---|
expression of a A328W/Y332A mutant BChE with improved cocaine hydrolase activity in transgenic Nicotiana benthamiana plants | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A328W/Y332A | the engineered recombinant cocaine hydrolase displays increased cocaine hydrolysis ability and increased affinity to the organophosphate pesticides paraoxon and malaoxon compared to the wild-type enzyme, overview | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chlorpyrifos-oxon | - |
Homo sapiens | |
diisopropylfluorophosphate | - |
Homo sapiens | |
malaoxon | - |
Homo sapiens | |
additional information | the engineered recombinant BChE mutant A328W/Y332A displays increased affinity to the organophosphate pesticides paraoxon and malaoxon compared to the wild-type enzyme, overview | Homo sapiens | |
neostigmine bromide | - |
Homo sapiens | |
paraoxon | - |
Homo sapiens | |
tetra(monoisopropyl) diphosphortetramide | i.e. iso-OMPA | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
cocaine | A328W/Y332A mutant BChE | Homo sapiens | |
0.434 | - |
cocaine | wild-type BChE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cocaine + H2O | Homo sapiens | - |
ecgonine methyl ester + benzoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cocaine + H2O | - |
Homo sapiens | ecgonine methyl ester + benzoate | - |
? | |
cocaine + H2O | cv. EC 3.1.1.1 | Homo sapiens | ecgonine methyl ester + benzoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BChE | - |
Homo sapiens |
butyrylcholinesterase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
386.8 | - |
cocaine | A328W/Y332A mutant BChE | Homo sapiens | |
418.3 | - |
cocaine | wild-type BChE | Homo sapiens |