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Literature summary for 3.1.1.8 extracted from
- Simplified procedures for purification and stabilization of human plasma butyrylcholinesterase (2004), Proc. Biochem., 39, 877-882.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| stabilized by glycerol, polythylene glycol, or ammonium sulfate, all best at 20% w/v | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| butyrylthiocholine | substrate inhibition above 10 mM | Homo sapiens |  |
| EDTA | inhibitory above 1 mM | Homo sapiens | |
| paraoxon | 50% inhibition at 0.0028 mM | Homo sapiens | |
| parathion | 50% inhibition at 0.022 mM | Homo sapiens | |
| propionylthiocholine | substrate inhibition above 10 mM | Homo sapiens | |
| tetra monoisopropyl pyrophosphomide | 50% inhibition at 0.055 mM | Homo sapiens | |
| Zn2+ | 1-2 mM, 10% activation, inhibitory above | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.018 | - |
butyrylthiocholine | pH 7.4, 30°C | Homo sapiens | |
| 0.083 | - |
acetylthiocholine | pH 7.4, 30°C | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | 1-2 mM, 10% activation, inhibitory above | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
x * 85000, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood plasma | - |
Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 30°C, 20% w/v ammonium sulfate, 12 weeks, 62% loss of activity | Homo sapiens |
| 30°C, 20% w/v glycerol, 12 weeks, 38% loss of activity | Homo sapiens |
| 30°C, 20% w/v polyethylene glycol 6000, 12 weeks, 20% loss of activity | Homo sapiens |
| 4°C, 20% w/v ammonium sulfate, 12 weeks, no loss of activity | Homo sapiens |
| 4°C, 20% w/v glycerol, 12 weeks, no loss of activity | Homo sapiens |
| 4°C, 20% w/v polyethylene glycol 6000, 12 weeks, no loss of activity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylthiocholine + H2O | - |
Homo sapiens | acetate + thiocholine | - |
? | |
| butyrylthiocholine + H2O | - |
Homo sapiens | thiocholine + butyrate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 85000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 42 | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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