Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.8 extracted from

  • Masson, P.; Bec, N.; Froment, M.T.; Nachon, F.; Balny, C.; Lockridge, O.; Schopfer, L.M.
    Rate-determining step of butyrylcholinesterase-catalyzed hydrolysis of benzoylcholine and benzoylthiocholine. Volumetric study of wild-type and D70G mutant behavior (2004), Eur. J. Biochem., 271, 1980-1990.
    View publication on PubMed
Search for more literature for 3.1.1.8

Protein Variants

Protein Variants Comment Organism
D70G 7-20fold increase in Km-value Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00295
-
 benzoylthiocholine wild-type, pH 7.4, 25°C Homo sapiens
0.00301
-
 Benzoylcholine wild-type, pH 7.4, 25°C Homo sapiens
0.0211
-
 Benzoylcholine mutant D70G, pH 7.4, 25°C Homo sapiens
0.0592
-
 benzoylthiocholine mutant D70G, pH 7.4, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoylcholine + H2O rate-limiting step for hydrolysis is deacylation, k3 Homo sapiens choline + benzoate
-
 ?
benzoylthiocholine + H2O rate-limiting step for hydrolysis is acylation, k2 Homo sapiens thiocholine + benzoate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.82
-
 benzoylthiocholine wild-type, pH 7.4, 25°C Homo sapiens
1.3
-
 benzoylthiocholine mutant D70G, pH 7.4, 25°C Homo sapiens
14.7
-
 Benzoylcholine wild-type, pH 7.4, 25°C Homo sapiens
15
-
 Benzoylcholine mutant D70G, pH 7.4, 25°C Homo sapiens