Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A328W | increased kcat | Homo sapiens |
A328W/Y332A | mutant reduces cocain burden in tissues, accelerates plasma clearance by 20fold and prevents cocaine-induced hyperactivity in mice | Homo sapiens |
Y332A | increased Km | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
(-)-cocaine | pH 7.4, A328W mutant | Homo sapiens | |
0.0045 | - |
(-)-cocaine | pH 7.4, wild-type enzyme | Homo sapiens | |
0.018 | - |
(-)-cocaine | pH 7.4, A328W/Y332A mutant | Homo sapiens | |
0.0245 | - |
(-)-cocaine | pH 7.4, Y332A mutant | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P06276 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-cocaine + H2O | - |
Homo sapiens | ? | - |
? | |
(-)-cocaine + H2O | - |
Homo sapiens | ? | - |
? | |
butyrylthiocholine + H2O | - |
Homo sapiens | thiocholine + butyrate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.068 | - |
(-)-cocaine | pH 7.4, wild-type enzyme | Homo sapiens | |
0.83 | - |
(-)-cocaine | pH 7.4, A328W mutant | Homo sapiens | |
1.83 | - |
(-)-cocaine | pH 7.4, Y332A mutant | Homo sapiens | |
2.6 | - |
(-)-cocaine | pH 7.4, A328W/Y332A mutant | Homo sapiens | |
2.94 | - |
(-)-cocaine | pH 7.4, Y332A mutant | Homo sapiens | |
6.08 | - |
(-)-cocaine | pH 7.4, A328W mutant | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | kcat with both cocaine stereoisomers is pH dependent in A328W/Y332A mutant, whereas that of wild-type enzyme is pH dependent only with the unnatural isomer, kcat increases with increasing pH | Homo sapiens |