Cloned (Comment) | Organism |
---|---|
expressed in CHO cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A539T | K-variant | Homo sapiens |
E497V/A539T | J-variant | Homo sapiens |
E797V | catalytic activity similar to wild-type enzyme | Homo sapiens |
General Stability | Organism |
---|---|
half-life of wild-type enzyme in culture: 1.41h, half-life of A539T mutant enzyme in culture: 1.55h, half-life of E497V/A539T mutant enzyme in culture: 1.49h | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
Benzoylcholine | wild-type and A539T mutant, pH 7, 25°C | Homo sapiens | |
0.009 | - |
Benzoylcholine | E497V/A539T mutant, pH 7, 25°C | Homo sapiens | |
0.011 | - |
Benzoylcholine | E497V mutant, pH 7, 25°C | Homo sapiens | |
0.017 | - |
butyrylthiocholine | A539T mutant, E497V/A539T mutant and E497V mutant, pH 7, 25°C | Homo sapiens | |
0.023 | - |
butyrylthiocholine | wild-type, pH 7, 25°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoylcholine + H2O | - |
Homo sapiens | choline + benzoate | - |
? | |
butyrylthiocholine + H2O | - |
Homo sapiens | thiocholine + butyrate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | in the presence of poly-L-proline | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
223 | - |
Benzoylcholine | E497V/A539T mutant, pH 7, 25°C | Homo sapiens | |
242 | - |
Benzoylcholine | wild-type, pH 7, 25°C | Homo sapiens | |
265 | - |
Benzoylcholine | A539T mutant, pH 7, 25°C | Homo sapiens | |
303 | - |
Benzoylcholine | E497V mutant, pH 7, 25°C | Homo sapiens | |
508 | - |
butyrylthiocholine | E497V/A539T mutant, pH 7, 25°C | Homo sapiens | |
542 | - |
butyrylthiocholine | A539T mutant and E497V mutant, pH 7, 25°C | Homo sapiens | |
565 | - |
butyrylthiocholine | wild-type, pH 7, 25°C | Homo sapiens |