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Literature summary for 3.1.1.8 extracted from

  • Masson, P.; Goldstein, B.N.; Debouzy, J.C.; Froment, M.T.; Lockridge, O.; Schopfer, L.M.
    Damped oscillatory hysteretic behaviour of butyrylcholinesterase with benzoylcholine as substrate (2004), Eur. J. Biochem., 271, 220-234.
    View publication on PubMed
Search for more literature for 3.1.1.8

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Protein Variants

Protein Variants Comment Organism
D79G increased Km Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 Benzoylcholine pH 7.4, 25°C, wild-type enzyme, kinetic mechanism Homo sapiens
0.0084
-
 benzoylthiocholine pH 7.4, 25°C, wild-type enzyme Homo sapiens
0.021
-
 Benzoylcholine pH 7.4, 25°C, D70G mutant Homo sapiens
0.0592
-
 benzoylthiocholine pH 7.4, 25°C, D70G mutant Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoylcholine + H2O
-
 Homo sapiens choline + benzoate
-
 ?
benzoylthiocholine + H2O
-
 Homo sapiens thiocholine + benzoate
-
 ?
butyrylthiocholine + H2O
-
 Homo sapiens thiocholine + butyrate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.3
-
 benzoylthiocholine pH 7.4, 25°C, wild-type enzyme Homo sapiens
21.6
-
 benzoylthiocholine pH 7.4, 25°C, D70G mutant Homo sapiens
245
-
 Benzoylcholine pH 7.4, 25°C, wild-type enzyme Homo sapiens
250
-
 Benzoylcholine pH 7.4, 25°C, D70G mutant Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 increasing activity with increasing pH Homo sapiens