Protein Variants | Comment | Organism |
---|---|---|
additional information | cells overexpressing the enzyme increase the efficacy of liver X receptor ligands on steroidogenic acute regulatory protein expression and steroid synthesis, suggesting enzyme-mediated steroidogenesis entails enhanced oxysterol production | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CAY10499 | - |
Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
a Leydig tumor strain | - |
Mus musculus MA-10 | - |
a Leydig tumor strain | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | reversible phosphorylation, the enzyme is phosphorylated at Ser660, Ser563, and Ser565, activation of the protein kinase A pathway, by a cAMP analogue dibutyryl cyclic AMP, enhances expression of the enzyme and its phosphorylationat Ser660 and Ser563, but not at Ser565 | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Leydig cell | - |
Mus musculus | - |
MA-10 cell | MA-10 mouse Leydig tumor cells | Mus musculus | - |
testis | - |
Mus musculus | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 81000-83000, phosphorylated enzyme, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
HSL | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Mus musculus |
Organism | Comment | Expression |
---|---|---|
Mus musculus | activation of the protein kinase A pathway, by a cAMP analogue dibutyryl cyclic AMP, enhances expression of the enzyme and its phosphorylation at Ser660 and Ser563, but not at Ser565, concomitant with increased enzyme activity. An increase in enzyme is correlated with the liver X receptor target genes, steroid receptor element-binding protein 1c, and ATP binding cassette transporter A1 | up |
General Information | Comment | Organism |
---|---|---|
malfunction | depletion of the enzyme affects lipoprotein-derived cellular cholesterol influx, diminishes the supply of cholesterol to the mitochondria, and results in the repression of steroidogenic acute regulatory protein and phospho-steroidogenic acute regulatory protein levels | Mus musculus |
additional information | cells deficient in liver X receptors exhibit decreased enzyme responsiveness | Mus musculus |
physiological function | the enzyme is primarily involved in catalyzing cholesteryl ester hydrolysis and plays an important role in the regulation of cAMP-mediated steroidogenic acute regulatory protein expression and steroidogenesis through modulation of liver X receptor pathways. The enzyme-dependent regulation of steroidogenesis predominantly involves liver X recetor signaling. Enzyme-mediated steroidogenesis entails enhanced oxysterol production. Hormonal control of the enzyme activity is primarily mediated by the phosphorylation of serine residues, Ser563, Ser659, and Ser660 | Mus musculus |