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Literature summary for 3.1.1.79 extracted from
- Structural and functional analyses of a bacterial homologue of hormone-sensitive lipase from a metagenomic library (2013), Acta Crystallogr. Sect. D, 69, 1726-1737.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | the requirement of dimerization for the function and stability of HSL-family enzymes focuses attention on the dimeric interface as a potential target for structure-aided drug design | uncultured bacterium |
| synthesis | the glutaraldehyde-medited crosslinked enzyme, showing increased stability, can be used as a biocatalyst in biotransformation processes | uncultured bacterium |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Est25, phylogenetic analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain XL1-Blue | uncultured bacterium |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant wild-type and selenomethionine-labeled enzyme, mixing of 0.001 ml of protein solution and 0.001 ml of reservoir solution, method optimization, X-ray diffraction structure determination and analysis at 1.49 A resolution | uncultured bacterium |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | crosslinking of purified recombinant enzyme by glutaraldehyde, the enzyme aggragates show increased stability compared to free enzyme | uncultured bacterium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured bacterium | Q4TZQ3 | identified from a metagenomic library by functional screening | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme by nickel affinity chromatography, gel filtration, and ultrafiltration | uncultured bacterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl acetate + H2O | - |
uncultured bacterium | 4-methylumbelliferol + acetate | - |
? |  |
| 4-nitrophenyl acetate + H2O | - |
uncultured bacterium | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
uncultured bacterium | 4-nitrophenol + butyrate | - |
? | |
| additional information | the enzyme shows broad substrate specificity, with a strong preference towards short-chain esters, e.g. 4-nitrophenyyl acetateand 4-nitrophenyyl butyrate, little activity is observed towards long-chain esters such as 4-nitrophenyl dodecanoate | uncultured bacterium | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | four molecules in the asymmetric unit of the enzyme are arranged as a dimer of dimers, the enzyme exhibits an alpha/beta-hydrolase fold consisting of a central beta-sheet enclosed by alpha-helices on both sides | uncultured bacterium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Est25 | - |
uncultured bacterium |
| HSL | - |
uncultured bacterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | uncultured bacterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | uncultured bacterium |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the enzyme contains a catalytic Ser201 and the other two catalytic residues Asp303 and His333, that perfectly match to corresponding residues in proteins within the HSL family | uncultured bacterium |
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