Cloned (Comment) | Organism |
---|---|
five C-terminally His-tagged human HSL variants are successfully cloned and expressed in Sf9 insect cells using the baculovirus/insect cell expression system | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S552A/S554A | variant AVA-SS, no effect on the activity | Homo sapiens |
S552E/S554E | variant AVA-AA, decreasing activity of human HSL against trioleoylglycerol, 1,2-dioleoyl-sn-glycerol and cholesteryl oleate substrates by 67%, 63% and 60%, respectively. The activity against p-nitrophenylbutyrate also decreases, but only by 30% compared to wild type HSL | Homo sapiens |
S554A | variant SVA-SS, resulted in a slight decrease in lipolytic activity | Homo sapiens |
S554E | variant SVE-SS, resulted in similar to wild type activity | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q05469 | - |
- |
Purification (Comment) | Organism |
---|---|
anion exchange chromatography followed by nickel affinity chromatography and dialysis | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.2 | - |
specific activity of the purified wild type enzyme against trioleoylglycerol | Homo sapiens |
2.3 | - |
specific activity of the purified wild type enzyme against cholesteryl oleate | Homo sapiens |
23 | - |
specific activity of the purified wild type enzyme against 1,2-dioleoyl-sn-glycerol | Homo sapiens |
50 | - |
specific activity of the purified wild type enzyme against p-nitrophenylbutyrate | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dioleoyl-sn-glycerol + H2O | - |
Homo sapiens | ? | - |
? | |
cholesteryl oleate + H2O | - |
Homo sapiens | ? | - |
? | |
p-nitrophenyl butyrate + H2O | - |
Homo sapiens | p-nitrophenol + butanoate | - |
? | |
trioleoylglycerol + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hormone-sensitive lipase | - |
Homo sapiens |
HSL | - |
Homo sapiens |