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Literature summary for 3.1.1.79 extracted from

  • Nam, K.H.; Kim, M.Y.; Kim, S.J.; Priyadarshi, A.; Lee, W.H.; Hwang, K.Y.
    Structural and functional analysis of a novel EstE5 belonging to the subfamily of hormone-sensitive lipase (2009), Biochem. Biophys. Res. Commun., 379, 553-556.
    View publication on PubMed
Search for more literature for 3.1.1.79

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in BL21(DE3) cells uncultured bacterium

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor-diffusion method uncultured bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.06
-
 p-nitrophenyl butyrate Vmax value is 493.6 microM/min uncultured bacterium

Organism

Organism UniProt Comment Textmining
uncultured bacterium Q0GMU2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 uncultured bacterium

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl acetate + H2O using various p-nitrophenyl esters as substrates. Enzyme efficiently hydrolyzes p-nitrophenyl acetate (75%), p-nitrophenyl butyrate (100%), p-nitrophenyl carproate (55%) and p-nitrophenyl caprylate (50%), a lower level of hydrolysis is observed for p-nitrophenyl decanoate (25%), p-nitrophenyl palmitate (2%) and p-nitrophenyl stearate (2%) uncultured bacterium ?
-
 ?
p-nitrophenyl butyrate + H2O using various p-nitrophenyl esters as substrates. Enzyme efficiently hydrolyzes p-nitrophenyl acetate (75%), p-nitrophenyl butyrate (100%), p-nitrophenyl carproate (55%) and p-nitrophenyl caprylate (50%), a lower level of hydrolysis is observed for p-nitrophenyl decanoate (25%), p-nitrophenyl palmitate (2%) and p-nitrophenyl stearate (2%) uncultured bacterium ?
-
 ?
p-nitrophenyl caproate + H2O using various p-nitrophenyl esters as substrates. Enzyme efficiently hydrolyzes p-nitrophenyl acetate (75%), p-nitrophenyl butyrate (100%), p-nitrophenyl carproate (55%) and p-nitrophenyl caprylate (50%), a lower level of hydrolysis is observed for p-nitrophenyl decanoate (25%), p-nitrophenyl palmitate (2%) and p-nitrophenyl stearate (2%) uncultured bacterium ?
-
 ?
p-nitrophenyl caprylate + H2O using various p-nitrophenyl esters as substrates. Enzyme efficiently hydrolyzes p-nitrophenyl acetate (75%), p-nitrophenyl butyrate (100%), p-nitrophenyl carproate (55%) and p-nitrophenyl caprylate (50%), a lower level of hydrolysis is observed for p-nitrophenyl decanoate (25%), p-nitrophenyl palmitate (2%) and p-nitrophenyl stearate (2%) uncultured bacterium ?
-
 ?
p-nitrophenyl decanoate + H2O using various p-nitrophenyl esters as substrates. Enzyme efficiently hydrolyzes p-nitrophenyl acetate (75%), p-nitrophenyl butyrate (100%), p-nitrophenyl carproate (55%) and p-nitrophenyl caprylate (50%), a lower level of hydrolysis is observed for p-nitrophenyl decanoate (25%), p-nitrophenyl palmitate (2%) and p-nitrophenyl stearate (2%) uncultured bacterium ?
-
 ?
p-nitrophenyl palmitate + H2O using various p-nitrophenyl esters as substrates. Enzyme efficiently hydrolyzes p-nitrophenyl acetate (75%), p-nitrophenyl butyrate (100%), p-nitrophenyl carproate (55%) and p-nitrophenyl caprylate (50%), a lower level of hydrolysis is observed for p-nitrophenyl decanoate (25%), p-nitrophenyl palmitate (2%) and p-nitrophenyl stearate (2%) uncultured bacterium ?
-
 ?
p-nitrophenyl stearate + H2O using various p-nitrophenyl esters as substrates. Enzyme efficiently hydrolyzes p-nitrophenyl acetate (75%), p-nitrophenyl butyrate (100%), p-nitrophenyl carproate (55%) and p-nitrophenyl caprylate (50%), a lower level of hydrolysis is observed for p-nitrophenyl decanoate (25%), p-nitrophenyl palmitate (2%) and p-nitrophenyl stearate (2%) uncultured bacterium ?
-
 ?

Synonyms

Synonyms Comment Organism
EstE5
-
 uncultured bacterium
EstE5 protein
-
 uncultured bacterium
hormone-sensitive lipase
-
 uncultured bacterium
HSL
-
 uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 uncultured bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 uncultured bacterium

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9 purified enzyme is broadly stable within a pH range of 5.5-9.0 uncultured bacterium