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Literature summary for 3.1.1.79 extracted from

  • Deb, C.; Daniel, J.; Sirakova, T.D.; Abomoelak, B.; Dubey, V.S.; Kolattukudy, P.E.
    A novel lipase belonging to the hormone-sensitive lipase family induced under starvation to utilize stored triacylglycerol in Mycobacterium tuberculosis (2006), J. Biol. Chem., 281, 3866-3875.
    View publication on PubMedView publication on EuropePMC
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Activating Compound

Activating Compound Comment Organism Structure
potassium acetate enhances activity Mycobacterium tuberculosis
SDS slight stimulation up to 2 mM Mycobacterium tuberculosis
Sodium acetate enhances activity Mycobacterium tuberculosis
Triton X-100 0.1%, stimulates Mycobacterium tuberculosis

General Stability

General Stability Organism
stable to 4 cycles of freezing at -20°C followed by thawing Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 50 mM, 47% inhibition Mycobacterium tuberculosis
CoCl2 10 mM, 34% inhibition Mycobacterium tuberculosis
MgCl2 50 mM, 52% inhibition Mycobacterium tuberculosis
MnCl2 50 mM, 45% inhibition Mycobacterium tuberculosis
SDS above 2 mM. Inhibition by SDS is partially reversed by Triton X-100 Mycobacterium tuberculosis
Triton X-100 above 0.1% Mycobacterium tuberculosis
Tween 20 inhibits from 0.1 to 2.0% Mycobacterium tuberculosis
ZnCl2 44% inhibition at 10 mM, 99% inhibition at 50 mM Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.57
-
 triolein
-
 Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
KCl enhances activity Mycobacterium tuberculosis
NaCl enhances activity Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mycobacterium tuberculosis LIPY may be responsible for the utilization of stored triglycerides during dormancy and reactivation of the pathogen Mycobacterium tuberculosis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycobacterium tuberculosis

Storage Stability

Storage Stability Organism
4°C, stable for 60 days Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information LIPY may be responsible for the utilization of stored triglycerides during dormancy and reactivation of the pathogen Mycobacterium tuberculosis Mycobacterium tuberculosis ?
-
 ?
triolein + H2O
-
 Mycobacterium tuberculosis dioleoylglycerol + oleate
-
 ?

Synonyms

Synonyms Comment Organism
LipY
-
 Mycobacterium tuberculosis
Rv3097c
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Mycobacterium tuberculosis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 25°C: about 90% of maximal activity, 50°C: about 40% of maximal activity Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
 Mycobacterium tuberculosis

pH Range

pH Minimum pH Maximum Comment Organism
7 10 pH 7: about 60% of maximal activity, pH 10.: about 50% of maximal activity Mycobacterium tuberculosis