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Literature summary for 3.1.1.79 extracted from

  • Tsujita,T.; Okuda, H.
    Hydrolysis of cholesteryl oleate liquid crystals by hormon-sensitive lipase (1993), J. Biochem., 113, 264-269.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphatidylcholine 0.075 mM, slight activation upon incorporation into cholestryl oleate liquid crystals Bos taurus
phosphatidylethanolamine 0.075 mM, approx. 6fold activation upon incorporation into cholestryl oleate liquid crystals Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
cholesteryl oleate cholesteryl oleate vesicles, 37°C, pH 6.8 Bos taurus
0.012
-
cholesteryl oleate cholesteryl oleate emulsion, 37°C, pH 6.8 Bos taurus
0.099
-
cholesteryl oleate liquid crystals of cholesteryl oleate, 37°C, pH 6.8 Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
adipose tissue
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesteryl oleate + H2O
-
Bos taurus cholesterol + oleate
-
?
p-nitrophenyl butyrate + H2O
-
Bos taurus p-nitrophenol + butanoate
-
?
triolein + H2O
-
Bos taurus dioleoylglycerol + oleate
-
?

Synonyms

Synonyms Comment Organism
HSL
-
Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
5 9 approx. 50% of maximal activity at pH 5.5 and pH 8.5, respectively Bos taurus