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Literature summary for 3.1.1.78 extracted from

  • Dogru, E.; Warzecha, H.; Seibel, F.; Haebel, S.; Lottspeich, F.; Stockigt, J.
    The gene encoding polyneuridine aldehyde esterase of monoterpenoid indole alkaloid biosynthesis in plants is an ortholog of the a/b hydrolase super family (2000), Eur. J. Biochem., 267, 1397-1406.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
fused with a C-terminal His-tag Rauvolfia serpentina Benth. ex Kurz

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.83
-
 polyneuridine aldehyde
-
 Rauvolfia serpentina Benth. ex Kurz

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 2 * 30000, SDS-PAGE Rauvolfia serpentina Benth. ex Kurz

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polyneuridine aldehyde + H2O Rauvolfia serpentina Benth. ex Kurz
-
 16-epivellosimine + CO2 + methanol
-
 ?

Organism

Organism UniProt Comment Textmining
Rauvolfia serpentina Benth. ex Kurz
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rauvolfia serpentina Benth. ex Kurz

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
polyneuridine aldehyde + H2O
-
 Rauvolfia serpentina Benth. ex Kurz 16-epivellosimine + CO2 + methanol
-
 ?
polyneuridine aldehyde + H2O intermediate is polyneuridine aldehyde acid Rauvolfia serpentina Benth. ex Kurz 16-epivellosimine + CO2 + methanol 16-epivellosimine reacts to vellosimine ?

Subunits

Subunits Comment Organism
homodimer 2 * 30000, SDS-PAGE Rauvolfia serpentina Benth. ex Kurz