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Literature summary for 3.1.1.78 extracted from
- Potential active-site residues in polyneuridine aldehyde esterase, a central enzyme of indole alkaloid biosynthesis, by modelling and site-directed mutagenesis (2002), Eur. J. Biochem., 269, 2889-2896.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli M15 | Rauvolfia serpentina Benth. ex Kurz |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C132A | 755% lower activity than wild-type | Rauvolfia serpentina Benth. ex Kurz |
| C170A | 80% lower activity than wild-type | Rauvolfia serpentina Benth. ex Kurz |
| C213S/G152Q | 35% lower activity than wild-type | Rauvolfia serpentina Benth. ex Kurz |
| C257A | 35% lower activity than wild-type | Rauvolfia serpentina Benth. ex Kurz |
| H17A | 97% lower activity than wild-type | Rauvolfia serpentina Benth. ex Kurz |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethyldicarbonate | 100% inhibition at 0.8 mM | Rauvolfia serpentina Benth. ex Kurz |  |
| HgCl2 | 100% inhibition at 0.2 mM | Rauvolfia serpentina Benth. ex Kurz | |
| L-chloro-3-[4-tosyl-amido]-7-amido-2-heptanone | 12% inhibition at 0.12 mM | Rauvolfia serpentina Benth. ex Kurz | |
| phenylmethanesulfonyl fluoride | 20% inhibition at 1 mM | Rauvolfia serpentina Benth. ex Kurz | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
2 * 30000, SDS-PAGE | Rauvolfia serpentina Benth. ex Kurz |
| 60000 | - |
gel filtration | Rauvolfia serpentina Benth. ex Kurz |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polyneuridine aldehyde + H2O | Rauvolfia serpentina Benth. ex Kurz | central enzyme of indole alkaloid biosynthesis | 16-epivellosimine + CO2 + methanol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rauvolfia serpentina Benth. ex Kurz | - |
Ser87, Asp216 and His244 form a catalytic triad of polyneuridine aldehyde esterase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rauvolfia serpentina Benth. ex Kurz |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polyneuridine aldehyde + H2O | - |
Rauvolfia serpentina Benth. ex Kurz | 16-epivellosimine + CO2 + methanol | - |
? | |
| polyneuridine aldehyde + H2O | central enzyme of indole alkaloid biosynthesis | Rauvolfia serpentina Benth. ex Kurz | 16-epivellosimine + CO2 + methanol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 30000, SDS-PAGE | Rauvolfia serpentina Benth. ex Kurz |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay conditions | Rauvolfia serpentina Benth. ex Kurz |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay conditions | Rauvolfia serpentina Benth. ex Kurz |
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Release 2023.1 (January 2023)
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