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Literature summary for 3.1.1.76 extracted from
- Molecular characterization of extracellular medium-chain-length poly(3-hydroxyalkanoate) depolymerase genes from Pseudomonas alcaligenes strains (2005), J. Microbiol., 43, 285-294.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene phaZ, DNA and amino acid sequence determination and analysis, expression and subcloning in Escherichia coli strain DH5alpha | Pseudomonas alcaligenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-hydroxymercuribenzoate | - |
Pseudomonas alcaligenes |  |
| Acetic anhydride | - |
Pseudomonas alcaligenes | |
| diisopropylfluorophosphate | - |
Pseudomonas alcaligenes | |
| EDTA | - |
Pseudomonas alcaligenes | |
| iodoacetamide | - |
Pseudomonas alcaligenes | |
| additional information | no inhibition by DTT; no inhibition by DTT, sodium azide, and iododacetamide | Pseudomonas alcaligenes | |
| N-bromosuccimide | - |
Pseudomonas alcaligenes | |
| N-bromosuccinimide | - |
Pseudomonas alcaligenes | |
| PMSF | - |
Pseudomonas alcaligenes | |
| Sodium azide | - |
Pseudomonas alcaligenes | |
| Triton X-100 | - |
Pseudomonas alcaligenes | |
| Tween 80 | - |
Pseudomonas alcaligenes | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pseudomonas alcaligenes | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
x * 28000, SDS-PAGE | Pseudomonas alcaligenes |
| 30323 | - |
x * 30323, amino acid sequence calculation | Pseudomonas alcaligenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(3-hydroxyalkanoate) + H2O | Pseudomonas alcaligenes | - |
oligo(3-hydroxyalkanoate) + 3-hydroxyalkanoate | - |
? | |
| poly(3-hydroxyalkanoate) + H2O | Pseudomonas alcaligenes | - |
oligo(3-hydroxalkanoate) | - |
? | |
| poly(3-hydroxyalkanoate) + H2O | Pseudomonas alcaligenes LB19 | - |
oligo(3-hydroxyalkanoate) + 3-hydroxyalkanoate | - |
? | |
| poly(3-hydroxyalkanoate) + H2O | Pseudomonas alcaligenes M4-7 | - |
oligo(3-hydroxalkanoate) | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas alcaligenes | Q6UFW4 | gene phaZ, precursor; gene phaZ | - |
| Pseudomonas alcaligenes | Q84C08 | gene phaZ, precursor; gene phaZ | - |
| Pseudomonas alcaligenes LB19 | Q6UFW4 | gene phaZ, precursor; gene phaZ | - |
| Pseudomonas alcaligenes M4-7 | Q84C08 | gene phaZ, precursor; gene phaZ | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from strain M4-7 | Pseudomonas alcaligenes |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| [oxycarbonyl[(R)-2-pentylethylene]]n + H2O = [oxycarbonyl[(R)-2-pentylethylene]]m + [oxycarbonyl[(R)-2-pentylethylene]]g | Asn111 and Ser110 function as oxyanions important in transition-state stabilization of the hydrolytic reaction | Pseudomonas alcaligenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl octanoate + H2O | - |
Pseudomonas alcaligenes | 4-nitrophenol + octanoate | - |
? | |
| 4-nitrophenyl octanoate + H2O | - |
Pseudomonas alcaligenes LB19 | 4-nitrophenol + octanoate | - |
? | |
| 4-nitrophenyl octanoate + H2O | - |
Pseudomonas alcaligenes M4-7 | 4-nitrophenol + octanoate | - |
? | |
| poly(3-hydroxyalkanoate) + H2O | - |
Pseudomonas alcaligenes | oligo(3-hydroxyalkanoate) + 3-hydroxyalkanoate | - |
? | |
| poly(3-hydroxyalkanoate) + H2O | - |
Pseudomonas alcaligenes LB19 | oligo(3-hydroxyalkanoate) + 3-hydroxyalkanoate | - |
? | |
| poly(3-hydroxyalkanoate) + H2O | - |
Pseudomonas alcaligenes | oligo(3-hydroxalkanoate) | - |
? | |
| poly(3-hydroxyalkanoate) + H2O | - |
Pseudomonas alcaligenes M4-7 | oligo(3-hydroxalkanoate) | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28000, SDS-PAGE | Pseudomonas alcaligenes |
| ? | x * 30323, amino acid sequence calculation | Pseudomonas alcaligenes |
| More | the enzyme contains a lipase box | Pseudomonas alcaligenes |
| More | the enzyme possesses a catalytic domain in the N-terminus, and a separate substrate binding domain in the C-terminus, the enzyme contains a lipase box | Pseudomonas alcaligenes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MCL-PHA depolymerase | - |
Pseudomonas alcaligenes |
| medium-chain-length poly(3-hydroxyalkanoate) depolymerase | - |
Pseudomonas alcaligenes |
| PhaZ | - |
Pseudomonas alcaligenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Pseudomonas alcaligenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Pseudomonas alcaligenes |
| 9 | - |
- |
Pseudomonas alcaligenes |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudomonas alcaligenes | amino acid sequence calculation | - |
5.7 |
| Pseudomonas alcaligenes | amino acid sequence calculation | - |
5.9 |
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