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Literature summary for 3.1.1.75 extracted from

  • Gangoiti, J.; Santos, M.; Prieto, M.A.; de la Mata, I.; Serra, J.L.; Llama, M.J.
    Characterization of a novel subgroup of extracellular medium-chain-length polyhydroxyalkanoate depolymerases from actinobacteria (2012), Appl. Environ. Microbiol., 78, 7229-7237.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene phaZSomSO2, DNA and amino acid sequence determination and analysis Streptomyces omiyaensis
gene phaZSroSL3, DNA and amino acid sequence determination and analysis Streptomyces roseolus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Streptomyces atratus
-
-
extracellular
-
Streptomyces anulatus
-
-
extracellular
-
Streptomyces omiyaensis
-
-
extracellular
-
Streptomyces beijiangensis
-
-
extracellular
-
Streptomyces pulveraceus
-
-
extracellular
-
Streptomyces roseolus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
native PAGE Streptomyces roseolus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Streptomyces roseolus the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview ?
-
?
additional information Streptomyces anulatus the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview ?
-
?
additional information Streptomyces omiyaensis the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview ?
-
?
additional information Streptomyces beijiangensis the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview ?
-
?
additional information Streptomyces pulveraceus the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview ?
-
?
additional information Streptomyces atratus the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate), poly(L-lactide), and poly(epsilon-caprolactone), substrate specificity in vivo, overview ?
-
?
additional information Streptomyces anulatus SL11 the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview ?
-
?
additional information Streptomyces omiyaensis SO2 CECT 7923 the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview ?
-
?
additional information Streptomyces roseolus SL3 CECT 7919 the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview ?
-
?
additional information Streptomyces atratus SL2 the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate), poly(L-lactide), and poly(epsilon-caprolactone), substrate specificity in vivo, overview ?
-
?
additional information Streptomyces pulveraceus W3 the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview ?
-
?
additional information Streptomyces beijiangensis SL15 the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview ?
-
?
poly(3-hydroxybutyrate) + H2O Streptomyces atratus
-
?
-
?
poly(3-hydroxybutyrate) + H2O Streptomyces anulatus
-
?
-
?
poly(3-hydroxybutyrate) + H2O Streptomyces omiyaensis
-
?
-
?
poly(3-hydroxybutyrate) + H2O Streptomyces beijiangensis
-
?
-
?
poly(3-hydroxybutyrate) + H2O Streptomyces pulveraceus
-
?
-
?
poly(3-hydroxybutyrate) + H2O Streptomyces roseolus
-
?
-
?
poly(3-hydroxybutyrate) + H2O Streptomyces anulatus SL11
-
?
-
?
poly(3-hydroxybutyrate) + H2O Streptomyces omiyaensis SO2 CECT 7923
-
?
-
?
poly(3-hydroxybutyrate) + H2O Streptomyces roseolus SL3 CECT 7919
-
?
-
?
poly(3-hydroxybutyrate) + H2O Streptomyces atratus SL2
-
?
-
?
poly(3-hydroxybutyrate) + H2O Streptomyces pulveraceus W3
-
?
-
?
poly(3-hydroxybutyrate) + H2O Streptomyces beijiangensis SL15
-
?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O Streptomyces atratus
-
?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O Streptomyces anulatus
-
?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O Streptomyces omiyaensis
-
?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O Streptomyces beijiangensis
-
?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O Streptomyces pulveraceus
-
?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O Streptomyces roseolus
-
?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O Streptomyces anulatus SL11
-
?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O Streptomyces omiyaensis SO2 CECT 7923
-
?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O Streptomyces roseolus SL3 CECT 7919
-
?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O Streptomyces atratus SL2
-
?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O Streptomyces pulveraceus W3
-
?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O Streptomyces beijiangensis SL15
-
?
-
?
poly(3-hydroxypropionate) + H2O Streptomyces atratus
-
?
-
?
poly(3-hydroxypropionate) + H2O Streptomyces anulatus
-
?
-
?
poly(3-hydroxypropionate) + H2O Streptomyces omiyaensis
-
?
-
?
poly(3-hydroxypropionate) + H2O Streptomyces roseolus
-
?
-
?
poly(3-hydroxypropionate) + H2O Streptomyces anulatus SL11
-
?
-
?
poly(3-hydroxypropionate) + H2O Streptomyces omiyaensis SO2 CECT 7923
-
?
-
?
poly(3-hydroxypropionate) + H2O Streptomyces roseolus SL3 CECT 7919
-
?
-
?
poly(3-hydroxypropionate) + H2O Streptomyces atratus SL2
-
?
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces anulatus
-
-
-
Streptomyces anulatus SL11
-
-
-
Streptomyces atratus
-
-
-
Streptomyces atratus SL2
-
-
-
Streptomyces beijiangensis
-
-
-
Streptomyces beijiangensis SL15
-
-
-
Streptomyces omiyaensis
-
isolated from soil, gene phaZSomSO2
-
Streptomyces omiyaensis SO2 CECT 7923
-
isolated from soil, gene phaZSomSO2
-
Streptomyces pulveraceus
-
-
-
Streptomyces pulveraceus W3
-
-
-
Streptomyces roseolus
-
isolated from sludge, gene phaZSroSL3
-
Streptomyces roseolus SL3 CECT 7919
-
isolated from sludge, gene phaZSroSL3
-

Purification (Commentary)

Purification (Comment) Organism
extracellular enzyme by gel filtration and adsorption chromatography Streptomyces omiyaensis
extracellular enzyme by gel filtration and adsorption chromatography Streptomyces roseolus

Source Tissue

Source Tissue Comment Organism Textmining
aerial mycelium
-
Streptomyces omiyaensis
-
aerial mycelium
-
Streptomyces roseolus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview Streptomyces roseolus ?
-
?
additional information the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview Streptomyces anulatus ?
-
?
additional information the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview Streptomyces omiyaensis ?
-
?
additional information the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview Streptomyces beijiangensis ?
-
?
additional information the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview Streptomyces pulveraceus ?
-
?
additional information the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate), poly(L-lactide), and poly(epsilon-caprolactone), substrate specificity in vivo, overview Streptomyces atratus ?
-
?
additional information the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview Streptomyces anulatus SL11 ?
-
?
additional information the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview Streptomyces omiyaensis SO2 CECT 7923 ?
-
?
additional information the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview Streptomyces roseolus SL3 CECT 7919 ?
-
?
additional information the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate), poly(L-lactide), and poly(epsilon-caprolactone), substrate specificity in vivo, overview Streptomyces atratus SL2 ?
-
?
additional information the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview Streptomyces pulveraceus W3 ?
-
?
additional information the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview Streptomyces beijiangensis SL15 ?
-
?
poly(3-hydroxybutyrate) + H2O
-
Streptomyces atratus ?
-
?
poly(3-hydroxybutyrate) + H2O
-
Streptomyces anulatus ?
-
?
poly(3-hydroxybutyrate) + H2O
-
Streptomyces omiyaensis ?
-
?
poly(3-hydroxybutyrate) + H2O
-
Streptomyces beijiangensis ?
-
?
poly(3-hydroxybutyrate) + H2O
-
Streptomyces pulveraceus ?
-
?
poly(3-hydroxybutyrate) + H2O
-
Streptomyces roseolus ?
-
?
poly(3-hydroxybutyrate) + H2O
-
Streptomyces anulatus SL11 ?
-
?
poly(3-hydroxybutyrate) + H2O
-
Streptomyces omiyaensis SO2 CECT 7923 ?
-
?
poly(3-hydroxybutyrate) + H2O
-
Streptomyces roseolus SL3 CECT 7919 ?
-
?
poly(3-hydroxybutyrate) + H2O
-
Streptomyces atratus SL2 ?
-
?
poly(3-hydroxybutyrate) + H2O
-
Streptomyces pulveraceus W3 ?
-
?
poly(3-hydroxybutyrate) + H2O
-
Streptomyces beijiangensis SL15 ?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O
-
Streptomyces atratus ?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O
-
Streptomyces anulatus ?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O
-
Streptomyces omiyaensis ?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O
-
Streptomyces beijiangensis ?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O
-
Streptomyces pulveraceus ?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O
-
Streptomyces roseolus ?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O
-
Streptomyces anulatus SL11 ?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O
-
Streptomyces omiyaensis SO2 CECT 7923 ?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O
-
Streptomyces roseolus SL3 CECT 7919 ?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O
-
Streptomyces atratus SL2 ?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O
-
Streptomyces pulveraceus W3 ?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O
-
Streptomyces beijiangensis SL15 ?
-
?
poly(3-hydroxypropionate) + H2O
-
Streptomyces atratus ?
-
?
poly(3-hydroxypropionate) + H2O
-
Streptomyces anulatus ?
-
?
poly(3-hydroxypropionate) + H2O
-
Streptomyces omiyaensis ?
-
?
poly(3-hydroxypropionate) + H2O
-
Streptomyces roseolus ?
-
?
poly(3-hydroxypropionate) + H2O
-
Streptomyces anulatus SL11 ?
-
?
poly(3-hydroxypropionate) + H2O
-
Streptomyces omiyaensis SO2 CECT 7923 ?
-
?
poly(3-hydroxypropionate) + H2O
-
Streptomyces roseolus SL3 CECT 7919 ?
-
?
poly(3-hydroxypropionate) + H2O
-
Streptomyces atratus SL2 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 28000, SDS-PAGE Streptomyces roseolus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Streptomyces atratus
30
-
assay at Streptomyces anulatus
30
-
assay at Streptomyces omiyaensis
30
-
assay at Streptomyces beijiangensis
30
-
assay at Streptomyces roseolus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Streptomyces roseolus
9.5
-
assay at Streptomyces atratus
9.5
-
assay at Streptomyces anulatus
9.5
-
assay at Streptomyces omiyaensis
9.5
-
assay at Streptomyces beijiangensis
9.5
-
assay at Streptomyces pulveraceus

pH Range

pH Minimum pH Maximum Comment Organism
8 10.5 60% of maximal activity within this range Streptomyces roseolus

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces roseolus isoelectric focusing
-
5.2

General Information

General Information Comment Organism
evolution enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview Streptomyces atratus
evolution enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview Streptomyces anulatus
evolution enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview Streptomyces omiyaensis
evolution enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview Streptomyces beijiangensis
evolution enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview Streptomyces pulveraceus
evolution enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview Streptomyces roseolus