Cloned (Comment) | Organism |
---|---|
gene phaZSomSO2, DNA and amino acid sequence determination and analysis | Streptomyces omiyaensis |
gene phaZSroSL3, DNA and amino acid sequence determination and analysis | Streptomyces roseolus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces atratus | - |
- |
extracellular | - |
Streptomyces anulatus | - |
- |
extracellular | - |
Streptomyces omiyaensis | - |
- |
extracellular | - |
Streptomyces beijiangensis | - |
- |
extracellular | - |
Streptomyces pulveraceus | - |
- |
extracellular | - |
Streptomyces roseolus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
native PAGE | Streptomyces roseolus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Streptomyces roseolus | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | ? | - |
? | |
additional information | Streptomyces anulatus | the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | ? | - |
? | |
additional information | Streptomyces omiyaensis | the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | ? | - |
? | |
additional information | Streptomyces beijiangensis | the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | ? | - |
? | |
additional information | Streptomyces pulveraceus | the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | ? | - |
? | |
additional information | Streptomyces atratus | the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate), poly(L-lactide), and poly(epsilon-caprolactone), substrate specificity in vivo, overview | ? | - |
? | |
additional information | Streptomyces anulatus SL11 | the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | ? | - |
? | |
additional information | Streptomyces omiyaensis SO2 CECT 7923 | the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | ? | - |
? | |
additional information | Streptomyces roseolus SL3 CECT 7919 | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | ? | - |
? | |
additional information | Streptomyces atratus SL2 | the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate), poly(L-lactide), and poly(epsilon-caprolactone), substrate specificity in vivo, overview | ? | - |
? | |
additional information | Streptomyces pulveraceus W3 | the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | ? | - |
? | |
additional information | Streptomyces beijiangensis SL15 | the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | ? | - |
? | |
poly(3-hydroxybutyrate) + H2O | Streptomyces atratus | - |
? | - |
? | |
poly(3-hydroxybutyrate) + H2O | Streptomyces anulatus | - |
? | - |
? | |
poly(3-hydroxybutyrate) + H2O | Streptomyces omiyaensis | - |
? | - |
? | |
poly(3-hydroxybutyrate) + H2O | Streptomyces beijiangensis | - |
? | - |
? | |
poly(3-hydroxybutyrate) + H2O | Streptomyces pulveraceus | - |
? | - |
? | |
poly(3-hydroxybutyrate) + H2O | Streptomyces roseolus | - |
? | - |
? | |
poly(3-hydroxybutyrate) + H2O | Streptomyces anulatus SL11 | - |
? | - |
? | |
poly(3-hydroxybutyrate) + H2O | Streptomyces omiyaensis SO2 CECT 7923 | - |
? | - |
? | |
poly(3-hydroxybutyrate) + H2O | Streptomyces roseolus SL3 CECT 7919 | - |
? | - |
? | |
poly(3-hydroxybutyrate) + H2O | Streptomyces atratus SL2 | - |
? | - |
? | |
poly(3-hydroxybutyrate) + H2O | Streptomyces pulveraceus W3 | - |
? | - |
? | |
poly(3-hydroxybutyrate) + H2O | Streptomyces beijiangensis SL15 | - |
? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Streptomyces atratus | - |
? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Streptomyces anulatus | - |
? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Streptomyces omiyaensis | - |
? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Streptomyces beijiangensis | - |
? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Streptomyces pulveraceus | - |
? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Streptomyces roseolus | - |
? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Streptomyces anulatus SL11 | - |
? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Streptomyces omiyaensis SO2 CECT 7923 | - |
? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Streptomyces roseolus SL3 CECT 7919 | - |
? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Streptomyces atratus SL2 | - |
? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Streptomyces pulveraceus W3 | - |
? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Streptomyces beijiangensis SL15 | - |
? | - |
? | |
poly(3-hydroxypropionate) + H2O | Streptomyces atratus | - |
? | - |
? | |
poly(3-hydroxypropionate) + H2O | Streptomyces anulatus | - |
? | - |
? | |
poly(3-hydroxypropionate) + H2O | Streptomyces omiyaensis | - |
? | - |
? | |
poly(3-hydroxypropionate) + H2O | Streptomyces roseolus | - |
? | - |
? | |
poly(3-hydroxypropionate) + H2O | Streptomyces anulatus SL11 | - |
? | - |
? | |
poly(3-hydroxypropionate) + H2O | Streptomyces omiyaensis SO2 CECT 7923 | - |
? | - |
? | |
poly(3-hydroxypropionate) + H2O | Streptomyces roseolus SL3 CECT 7919 | - |
? | - |
? | |
poly(3-hydroxypropionate) + H2O | Streptomyces atratus SL2 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces anulatus | - |
- |
- |
Streptomyces anulatus SL11 | - |
- |
- |
Streptomyces atratus | - |
- |
- |
Streptomyces atratus SL2 | - |
- |
- |
Streptomyces beijiangensis | - |
- |
- |
Streptomyces beijiangensis SL15 | - |
- |
- |
Streptomyces omiyaensis | - |
isolated from soil, gene phaZSomSO2 | - |
Streptomyces omiyaensis SO2 CECT 7923 | - |
isolated from soil, gene phaZSomSO2 | - |
Streptomyces pulveraceus | - |
- |
- |
Streptomyces pulveraceus W3 | - |
- |
- |
Streptomyces roseolus | - |
isolated from sludge, gene phaZSroSL3 | - |
Streptomyces roseolus SL3 CECT 7919 | - |
isolated from sludge, gene phaZSroSL3 | - |
Purification (Comment) | Organism |
---|---|
extracellular enzyme by gel filtration and adsorption chromatography | Streptomyces omiyaensis |
extracellular enzyme by gel filtration and adsorption chromatography | Streptomyces roseolus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
aerial mycelium | - |
Streptomyces omiyaensis | - |
aerial mycelium | - |
Streptomyces roseolus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | Streptomyces roseolus | ? | - |
? | |
additional information | the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | Streptomyces anulatus | ? | - |
? | |
additional information | the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | Streptomyces omiyaensis | ? | - |
? | |
additional information | the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | Streptomyces beijiangensis | ? | - |
? | |
additional information | the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | Streptomyces pulveraceus | ? | - |
? | |
additional information | the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate), poly(L-lactide), and poly(epsilon-caprolactone), substrate specificity in vivo, overview | Streptomyces atratus | ? | - |
? | |
additional information | the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | Streptomyces anulatus SL11 | ? | - |
? | |
additional information | the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | Streptomyces omiyaensis SO2 CECT 7923 | ? | - |
? | |
additional information | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | Streptomyces roseolus SL3 CECT 7919 | ? | - |
? | |
additional information | the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate), poly(L-lactide), and poly(epsilon-caprolactone), substrate specificity in vivo, overview | Streptomyces atratus SL2 | ? | - |
? | |
additional information | the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | Streptomyces pulveraceus W3 | ? | - |
? | |
additional information | the enzyme is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), EC 3.1.1.76, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | Streptomyces beijiangensis SL15 | ? | - |
? | |
poly(3-hydroxybutyrate) + H2O | - |
Streptomyces atratus | ? | - |
? | |
poly(3-hydroxybutyrate) + H2O | - |
Streptomyces anulatus | ? | - |
? | |
poly(3-hydroxybutyrate) + H2O | - |
Streptomyces omiyaensis | ? | - |
? | |
poly(3-hydroxybutyrate) + H2O | - |
Streptomyces beijiangensis | ? | - |
? | |
poly(3-hydroxybutyrate) + H2O | - |
Streptomyces pulveraceus | ? | - |
? | |
poly(3-hydroxybutyrate) + H2O | - |
Streptomyces roseolus | ? | - |
? | |
poly(3-hydroxybutyrate) + H2O | - |
Streptomyces anulatus SL11 | ? | - |
? | |
poly(3-hydroxybutyrate) + H2O | - |
Streptomyces omiyaensis SO2 CECT 7923 | ? | - |
? | |
poly(3-hydroxybutyrate) + H2O | - |
Streptomyces roseolus SL3 CECT 7919 | ? | - |
? | |
poly(3-hydroxybutyrate) + H2O | - |
Streptomyces atratus SL2 | ? | - |
? | |
poly(3-hydroxybutyrate) + H2O | - |
Streptomyces pulveraceus W3 | ? | - |
? | |
poly(3-hydroxybutyrate) + H2O | - |
Streptomyces beijiangensis SL15 | ? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | - |
Streptomyces atratus | ? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | - |
Streptomyces anulatus | ? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | - |
Streptomyces omiyaensis | ? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | - |
Streptomyces beijiangensis | ? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | - |
Streptomyces pulveraceus | ? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | - |
Streptomyces roseolus | ? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | - |
Streptomyces anulatus SL11 | ? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | - |
Streptomyces omiyaensis SO2 CECT 7923 | ? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | - |
Streptomyces roseolus SL3 CECT 7919 | ? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | - |
Streptomyces atratus SL2 | ? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | - |
Streptomyces pulveraceus W3 | ? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | - |
Streptomyces beijiangensis SL15 | ? | - |
? | |
poly(3-hydroxypropionate) + H2O | - |
Streptomyces atratus | ? | - |
? | |
poly(3-hydroxypropionate) + H2O | - |
Streptomyces anulatus | ? | - |
? | |
poly(3-hydroxypropionate) + H2O | - |
Streptomyces omiyaensis | ? | - |
? | |
poly(3-hydroxypropionate) + H2O | - |
Streptomyces roseolus | ? | - |
? | |
poly(3-hydroxypropionate) + H2O | - |
Streptomyces anulatus SL11 | ? | - |
? | |
poly(3-hydroxypropionate) + H2O | - |
Streptomyces omiyaensis SO2 CECT 7923 | ? | - |
? | |
poly(3-hydroxypropionate) + H2O | - |
Streptomyces roseolus SL3 CECT 7919 | ? | - |
? | |
poly(3-hydroxypropionate) + H2O | - |
Streptomyces atratus SL2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28000, SDS-PAGE | Streptomyces roseolus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces atratus |
30 | - |
assay at | Streptomyces anulatus |
30 | - |
assay at | Streptomyces omiyaensis |
30 | - |
assay at | Streptomyces beijiangensis |
30 | - |
assay at | Streptomyces roseolus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Streptomyces roseolus |
9.5 | - |
assay at | Streptomyces atratus |
9.5 | - |
assay at | Streptomyces anulatus |
9.5 | - |
assay at | Streptomyces omiyaensis |
9.5 | - |
assay at | Streptomyces beijiangensis |
9.5 | - |
assay at | Streptomyces pulveraceus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10.5 | 60% of maximal activity within this range | Streptomyces roseolus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptomyces roseolus | isoelectric focusing | - |
5.2 |
General Information | Comment | Organism |
---|---|---|
evolution | enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview | Streptomyces atratus |
evolution | enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview | Streptomyces anulatus |
evolution | enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview | Streptomyces omiyaensis |
evolution | enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview | Streptomyces beijiangensis |
evolution | enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview | Streptomyces pulveraceus |
evolution | enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview | Streptomyces roseolus |