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Literature summary for 3.1.1.75 extracted from

  • Garcia-Hidalgo, J.; Hormigo, D.; Prieto, M.A.; Arroyo, M.; de la Mata, I.
    Extracellular production of Streptomyces exfoliatus poly(3-hydroxybutyrate) depolymerase in Rhodococcus sp. T104: determination of optimal biocatalyst conditions (2012), Appl. Microbiol. Biotechnol., 93, 1975-1988.
    View publication on PubMed
Search for more literature for 3.1.1.75

Cloned(Commentary)

Cloned (Comment) Organism
gene phaZSex, expression in Rhodococcus sp. strain T104 as an extracellular active enzyme, subcloning in Escherichia coli strain DH5alpha, expression in Escherichia coli strain BL21 (DE3) Streptomyces exfoliatus

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Streptomyces exfoliatus
Mn2+
-
 Streptomyces exfoliatus
NaCl complete inhibition at 3 M Streptomyces exfoliatus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptomyces exfoliatus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Streptomyces exfoliatus
Mg2+ activates Streptomyces exfoliatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly(3-hydroxybutyrate) + H2O Streptomyces exfoliatus
-
 ?
-
 ?
poly(3-hydroxybutyrate) + H2O Streptomyces exfoliatus DSM Z 41693
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces exfoliatus
-
 gene phaZSex
-
Streptomyces exfoliatus DSM Z 41693
-
 gene phaZSex
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Rhodococcus sp. strain T104 cell culture medium to homogeneity Streptomyces exfoliatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Streptomyces exfoliatus 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O
-
 Streptomyces exfoliatus DSM Z 41693 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Streptomyces exfoliatus 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Streptomyces exfoliatus DSM Z 41693 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl valerate + H2O
-
 Streptomyces exfoliatus 4-nitrophenol + valerate
-
 ?
4-nitrophenyl valerate + H2O
-
 Streptomyces exfoliatus DSM Z 41693 4-nitrophenol + valerate
-
 ?
poly(3-hydroxybutyrate) + H2O
-
 Streptomyces exfoliatus ?
-
 ?
poly(3-hydroxybutyrate) + H2O
-
 Streptomyces exfoliatus DSM Z 41693 ?
-
 ?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O with 5 or 12 mol% 3-hydroxyvalerate content Streptomyces exfoliatus ?
-
 ?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O with 5 or 12 mol% 3-hydroxyvalerate content Streptomyces exfoliatus DSM Z 41693 ?
-
 ?

Synonyms

Synonyms Comment Organism
PHB depolymerase
-
 Streptomyces exfoliatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Streptomyces exfoliatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 unfolding is observed at 55.8°C in a first stage Streptomyces exfoliatus
40
-
 the purified enzyme is highly stable at 40°C Streptomyces exfoliatus
70
-
 the purified enzyme is completely inactivated after 90 min Streptomyces exfoliatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Streptomyces exfoliatus