Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.75 extracted from

  • Takaku, H.; Kimoto, A.; Kodaira, S.; Nashimoto, M.; Takagi, M.
    Isolation of a Gram-positive poly(3-hydroxybutyrate) (PHB)-degrading bacterium from compost, and cloning and characterization of a gene encoding PHB depolymerase of Bacillus megaterium N-18-25-9 (2006), FEMS Microbiol. Lett., 264, 152-159.
    View publication on PubMed
Search for more literature for 3.1.1.75

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Priestia megaterium

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol 70% inhibition at 1 mM and 90% inhibition at 10 mM Priestia megaterium
KCl 100 mM, 75% inhibition Priestia megaterium
Phenylmethylsulfonylfluoride 10% inhibition at 1 mM and 70% inhibition at 10 mM Priestia megaterium
SDS almost complete inhibition at 0.1% Priestia megaterium
Triton X-100 almost complete inhibition at 0.1% Priestia megaterium
Tween 20 amlnost complete inhibition at 0.1% Priestia megaterium

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Priestia megaterium
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ depolymerase activity drastically increases in the presence of 1-100mM Ca2+ Priestia megaterium
K+ up to 100 mM, stimulation of depolymerase activity Priestia megaterium
Mg2+ up to 10 mM, stimulation of depolymerase activity Priestia megaterium
Na+ up to 100 mM, stimulation of depolymerase activity Priestia megaterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly[(R)-3-hydroxybutanoate]n + H2O Priestia megaterium the addition of PHB to the medium leads to a sixfold increase in PhaZBm mRNA, while the presence of glucose represses phaZBm expression poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O Priestia megaterium N-18-25-9 the addition of PHB to the medium leads to a sixfold increase in PhaZBm mRNA, while the presence of glucose represses phaZBm expression poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Organism

Organism UniProt Comment Textmining
Priestia megaterium A0A8W8 N-18-25-9
-
Priestia megaterium N-18-25-9 A0A8W8 N-18-25-9
-

Purification (Commentary)

Purification (Comment) Organism
expression in Escherichia coli Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl acetate + H2O
-
 Priestia megaterium p-nitrophenol + acetate
-
 ?
p-nitrophenyl acetate + H2O
-
 Priestia megaterium N-18-25-9 p-nitrophenol + acetate
-
 ?
p-nitrophenyl butyrate + H2O
-
 Priestia megaterium p-nitrophenol + butyrate
-
 ?
p-nitrophenyl butyrate + H2O
-
 Priestia megaterium N-18-25-9 p-nitrophenol + butyrate
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O the addition of PHB to the medium leads to a sixfold increase in PhaZBm mRNA, while the presence of glucose represses phaZBm expression Priestia megaterium poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O the addition of PHB to the medium leads to a sixfold increase in PhaZBm mRNA, while the presence of glucose represses phaZBm expression Priestia megaterium N-18-25-9 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Synonyms

Synonyms Comment Organism
PhaZBm
-
 Priestia megaterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Priestia megaterium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 75 50°C: about 50% of maximal activity, 75°C: about 50% of maximal activity Priestia megaterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Priestia megaterium

pH Range

pH Minimum pH Maximum Comment Organism
8 10 pH 8.0: 70% of maximal activity, pH 10.0: about 85% of maximal activity Priestia megaterium