Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 10 mM, 38% inhibition | Streptomyces sp. | |
Aprotinin | 10 mM, 26% inhibition | Streptomyces sp. | |
EDTA | 10 mM, 27% inhibition | Streptomyces sp. | |
Fe2+ | 10 mM, 17% inhibition | Streptomyces sp. | |
Mn2+ | 10 mM, 17% inhibition | Streptomyces sp. | |
Ni2+ | 10 mM, 28% inhibition | Streptomyces sp. | |
Plysurf A210G | 100 mg/l, 48% inhibition | Streptomyces sp. | |
PMSF | 10 mM, 72% inhibition | Streptomyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 10 mM, 1.29fold stimulation | Streptomyces sp. | |
Cu2+ | 10 mM, 1.65fold stimulation | Streptomyces sp. | |
Zn2+ | 10 mM, 1.19fold stimulation | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
1 * 41000, SDS-PAGE | Streptomyces sp. |
43000 | - |
gel filtration | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | poly(3-hydroxybutyrate-co-3-hydroxyvalerate) pof MW 43000 Da and 42000 Da | Streptomyces sp. | ? | - |
? | |
poly(beta-propiolactone) + H2O | - |
Streptomyces sp. | ? | - |
? | |
poly[(R)-3-hydroxybutanoate]n + H2O | degradation mainly to D-3-hydroxybutyric acid monomer | Streptomyces sp. | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41000, SDS-PAGE | Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Streptomyces sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | 40°C: about 30% of maximal activity, 70°C: about 30% of maximal activity | Streptomyces sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min, stable at | Streptomyces sp. |
60 | - |
60 in, 40% loss of activity | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Streptomyces sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | pH 6.0: about 75% of maximal activity, pH 9.5: about 60% of maximal activity | Streptomyces sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | 12 h, stable | Streptomyces sp. |