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Literature summary for 3.1.1.75 extracted from

  • Calabia, B.P.; Tokiwa, Y.
    A novel PHB depolymerase from a thermophilic Streptomyces sp. (2006), Biotechnol. Lett., 28, 383-388.
    View publication on PubMed
Search for more literature for 3.1.1.75

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 10 mM, 38% inhibition Streptomyces sp.
Aprotinin 10 mM, 26% inhibition Streptomyces sp.
EDTA 10 mM, 27% inhibition Streptomyces sp.
Fe2+ 10 mM, 17% inhibition Streptomyces sp.
Mn2+ 10 mM, 17% inhibition Streptomyces sp.
Ni2+ 10 mM, 28% inhibition Streptomyces sp.
Plysurf A210G 100 mg/l, 48% inhibition Streptomyces sp.
PMSF 10 mM, 72% inhibition Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 10 mM, 1.29fold stimulation Streptomyces sp.
Cu2+ 10 mM, 1.65fold stimulation Streptomyces sp.
Zn2+ 10 mM, 1.19fold stimulation Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 1 * 41000, SDS-PAGE Streptomyces sp.
43000
-
 gel filtration Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O poly(3-hydroxybutyrate-co-3-hydroxyvalerate) pof MW 43000 Da and 42000 Da Streptomyces sp. ?
-
 ?
poly(beta-propiolactone) + H2O
-
 Streptomyces sp. ?
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O degradation mainly to D-3-hydroxybutyric acid monomer Streptomyces sp. poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 41000, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Streptomyces sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 40°C: about 30% of maximal activity, 70°C: about 30% of maximal activity Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, stable at Streptomyces sp.
60
-
 60 in, 40% loss of activity Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 pH 6.0: about 75% of maximal activity, pH 9.5: about 60% of maximal activity Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8.5 12 h, stable Streptomyces sp.