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Literature summary for 3.1.1.75 extracted from

  • Han, J.S.; Kim, M.N.
    Purification and characterization of extracellular poly(3-hydroxybutyrate) depolymerase from Penicillium simplicissimum LAR13 (2002), J. Microbiol., 40, 20-25.
No PubMed abstract available
Search for more literature for 3.1.1.75

Inhibitors

Inhibitors Comment Organism Structure
diazo-DL-norleucine methyl ester 97% inhibition at 25 mM Penicillium simplicissimum
dithiothreitol complete inhibition at 5 mM Penicillium simplicissimum
Fe2+ 56% inhibition at 1 mM, almost complete inhibition at 4 mM Penicillium simplicissimum
Mercuric chloride complete inhibition at 10 mM Penicillium simplicissimum
phenylmethanesulfonyl fluoride 42% inhibition at 10 mM Penicillium simplicissimum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Penicillium simplicissimum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 SDS-PAGE Penicillium simplicissimum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly[(R)-3-hydroxybutanoate]n + H2O Penicillium simplicissimum
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O Penicillium simplicissimum LAR13
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Organism

Organism UniProt Comment Textmining
Penicillium simplicissimum
-
 LAR13
-
Penicillium simplicissimum LAR13
-
 LAR13
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein less than 5% carbohydrate content Penicillium simplicissimum

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, single chromatography step Penicillium simplicissimum

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
 Penicillium simplicissimum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 1 unit defined as the amount of protein required to decrease the value of A650 by 1.0 per minute, 27.5 U/mg Penicillium simplicissimum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly[(R)-3-hydroxybutanoate]n + H2O
-
 Penicillium simplicissimum poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O
-
 Penicillium simplicissimum LAR13 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 36000, SDS-PAGE Penicillium simplicissimum

Synonyms

Synonyms Comment Organism
PHB depolymerase
-
 Penicillium simplicissimum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Penicillium simplicissimum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable below for 30 min Penicillium simplicissimum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Penicillium simplicissimum

pH Range

pH Minimum pH Maximum Comment Organism
2 8
-
 Penicillium simplicissimum

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 10
-
 Penicillium simplicissimum