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Literature summary for 3.1.1.75 extracted from

  • Abe, T.; Kobayashi, T.; Saito, T.
    Properties of a novel intracellular poly(3-hydroxybutyrate) depolymerase with high specific activity (PhaZd) in Wautersia eutropha H16 (2005), J. Bacteriol., 187, 6982-6990.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
EDTA at 1 mM 130% activity, at 10 mM 140% activity Cupriavidus necator
Triton X-100 at 0.001% (w/v) 120% activity Cupriavidus necator

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Cupriavidus necator

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 at 10 mM 39% inhibition Cupriavidus necator
diisopropylfluorophosphate at 10 microM 53% inhibition, at 100 microM total loss of activity Cupriavidus necator
dithiothreitol at 0.1 mM 86% inhibition, complete inhibition at 1 mM Cupriavidus necator
MgCl2 slight inhibition 1 mM, 57% inhibition at 10 mM Cupriavidus necator
additional information no inhibition by phenylmethylsulfonyl fluoride Cupriavidus necator
p-(chloromercuri)benzoic acid no inhibition below 1 mM Cupriavidus necator
SDS at 0.01% (w/v) 28% inhibition, at 0.1% complete inhibition Cupriavidus necator
Triton X-100 at 0.1% 57% inhibition, at 1% 64% inhibition Cupriavidus necator

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular partially at poly(3-hydroxybutyrate) bodies and in the cytosol Cupriavidus necator 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39168
-
1 * 39168, SDS-PAGE Cupriavidus necator
39170
-
deduced from sequence, SDS-PAGE Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly[(R)-3-hydroxybutanoate]n + H2O Cupriavidus necator specific for amorphous poly(3-hydroxybutyrate) granules poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
?
poly[(R)-3-hydroxybutanoate]n + H2O Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 specific for amorphous poly(3-hydroxybutyrate) granules poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
?

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tag fusion, Ni-affinity chromatography Cupriavidus necator

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Cupriavidus necator
-

Storage Stability

Storage Stability Organism
-20°C, 20 mM Tris-HCl pH 8.0 50% glycerol, sable for more than 3 months Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl acetate + H2O
-
Cupriavidus necator p-nitrophenol + acetate
-
?
p-nitrophenyl acetate + H2O
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 p-nitrophenol + acetate
-
?
p-nitrophenyl butyrate + H2O
-
Cupriavidus necator p-nitrophenol + butyrate
-
?
p-nitrophenyl butyrate + H2O
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 p-nitrophenol + butyrate
-
?
poly[(R)-3-hydroxybutanoate]n + H2O specific for amorphous poly(3-hydroxybutyrate) granules Cupriavidus necator poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
?
poly[(R)-3-hydroxybutanoate]n + H2O specific for amorphous poly(3-hydroxybutyrate) granules, weak activity on 3-hydroxybutyrate dimers and trimers, no activity on tetramers or pentamers Cupriavidus necator poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
?
poly[(R)-3-hydroxybutanoate]n + H2O specific for amorphous poly(3-hydroxybutyrate) granules Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
?
poly[(R)-3-hydroxybutanoate]n + H2O specific for amorphous poly(3-hydroxybutyrate) granules, weak activity on 3-hydroxybutyrate dimers and trimers, no activity on tetramers or pentamers Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
?

Subunits

Subunits Comment Organism
monomer 1 * 39168, SDS-PAGE Cupriavidus necator

Synonyms

Synonyms Comment Organism
PhaZd
-
Cupriavidus necator
poly(3-hydroxybutyrate) depolymerase
-
Cupriavidus necator

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 30
-
Cupriavidus necator

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
stable for 10 min, at 40°C 80% activity is lost after 10 min, above 50°C all activity is lost Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9 in Tris-HCl buffer Cupriavidus necator