Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | at 1 mM 130% activity, at 10 mM 140% activity | Cupriavidus necator | |
Triton X-100 | at 0.001% (w/v) 120% activity | Cupriavidus necator |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Cupriavidus necator |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | at 10 mM 39% inhibition | Cupriavidus necator | |
diisopropylfluorophosphate | at 10 microM 53% inhibition, at 100 microM total loss of activity | Cupriavidus necator | |
dithiothreitol | at 0.1 mM 86% inhibition, complete inhibition at 1 mM | Cupriavidus necator | |
MgCl2 | slight inhibition 1 mM, 57% inhibition at 10 mM | Cupriavidus necator | |
additional information | no inhibition by phenylmethylsulfonyl fluoride | Cupriavidus necator | |
p-(chloromercuri)benzoic acid | no inhibition below 1 mM | Cupriavidus necator | |
SDS | at 0.01% (w/v) 28% inhibition, at 0.1% complete inhibition | Cupriavidus necator | |
Triton X-100 | at 0.1% 57% inhibition, at 1% 64% inhibition | Cupriavidus necator |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | partially at poly(3-hydroxybutyrate) bodies and in the cytosol | Cupriavidus necator | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39168 | - |
1 * 39168, SDS-PAGE | Cupriavidus necator |
39170 | - |
deduced from sequence, SDS-PAGE | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly[(R)-3-hydroxybutanoate]n + H2O | Cupriavidus necator | specific for amorphous poly(3-hydroxybutyrate) granules | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
poly[(R)-3-hydroxybutanoate]n + H2O | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | specific for amorphous poly(3-hydroxybutyrate) granules | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-tag fusion, Ni-affinity chromatography | Cupriavidus necator |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Cupriavidus necator | - |
Storage Stability | Organism |
---|---|
-20°C, 20 mM Tris-HCl pH 8.0 50% glycerol, sable for more than 3 months | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl acetate + H2O | - |
Cupriavidus necator | p-nitrophenol + acetate | - |
? | |
p-nitrophenyl acetate + H2O | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | p-nitrophenol + acetate | - |
? | |
p-nitrophenyl butyrate + H2O | - |
Cupriavidus necator | p-nitrophenol + butyrate | - |
? | |
p-nitrophenyl butyrate + H2O | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | p-nitrophenol + butyrate | - |
? | |
poly[(R)-3-hydroxybutanoate]n + H2O | specific for amorphous poly(3-hydroxybutyrate) granules | Cupriavidus necator | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
poly[(R)-3-hydroxybutanoate]n + H2O | specific for amorphous poly(3-hydroxybutyrate) granules, weak activity on 3-hydroxybutyrate dimers and trimers, no activity on tetramers or pentamers | Cupriavidus necator | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
poly[(R)-3-hydroxybutanoate]n + H2O | specific for amorphous poly(3-hydroxybutyrate) granules | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
poly[(R)-3-hydroxybutanoate]n + H2O | specific for amorphous poly(3-hydroxybutyrate) granules, weak activity on 3-hydroxybutyrate dimers and trimers, no activity on tetramers or pentamers | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 39168, SDS-PAGE | Cupriavidus necator |
Synonyms | Comment | Organism |
---|---|---|
PhaZd | - |
Cupriavidus necator |
poly(3-hydroxybutyrate) depolymerase | - |
Cupriavidus necator |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 30 | - |
Cupriavidus necator |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
stable for 10 min, at 40°C 80% activity is lost after 10 min, above 50°C all activity is lost | Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | in Tris-HCl buffer | Cupriavidus necator |