Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ApdA | without only reduced hydrolysis varying between 5 to 20% of the rate with activation | Rhodospirillum rubrum | |
Trypsin | without only reduced hydrolysis varying between 5 to 20% of the rate with activation | Rhodospirillum rubrum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | strong inhibition at 4 mM | Rhodospirillum rubrum | |
diisopropylfluorophosphate | almost complete inhibition at 1 mM | Rhodospirillum rubrum | |
dithiothreitol | strong inhibition at 4 mM | Rhodospirillum rubrum | |
EDTA | 79% activity at 1 mM, 20% activity at 20 mM | Rhodospirillum rubrum | |
SDS | almost complete inhibition at 0.1% | Rhodospirillum rubrum | |
Triton X-100 | almost complete inhibition at 0.1% | Rhodospirillum rubrum | |
Tween 20 | almost complete inhibition at 0.1% | Rhodospirillum rubrum | |
Tween 80 | almost complete inhibition at 0.1% | Rhodospirillum rubrum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Rhodospirillum rubrum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | increase of activity to 130% at 1 mM, but decrease to 75% at 5 mM and to 40% at 10 mM | Rhodospirillum rubrum | |
MgCl2 | increase of activity to 140% at 1 mM, but decrease to 75% at 5 mM and to 35% at 10 mM | Rhodospirillum rubrum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35247 | - |
1 * 35247, SDS-PAGE | Rhodospirillum rubrum |
35250 | - |
MALDI-TOF | Rhodospirillum rubrum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly[(R)-3-hydroxybutanoate]n + H2O | Rhodospirillum rubrum | - |
poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodospirillum rubrum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, two chromatograpphy steps | Rhodospirillum rubrum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Rhodospirillum rubrum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
74 | - |
74000 U/mg, 1 U is defined as the release of 1 nmol p-nitrophenol from p-nitrophenyl phosphate per minute | Rhodospirillum rubrum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly[(R)-3-hydroxybutanoate]n + H2O | - |
Rhodospirillum rubrum | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
poly[(R)-3-hydroxybutanoate]n + H2O | highly specific for oligomers or polymers of 3-hydroxybutyrate in the (R) configuration | Rhodospirillum rubrum | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35247, SDS-PAGE | Rhodospirillum rubrum |
Synonyms | Comment | Organism |
---|---|---|
PhaZ1 | - |
Rhodospirillum rubrum |
PHB depolymerase | - |
Rhodospirillum rubrum |
poly(3-hydroxybutyrate) depolymerase | - |
Rhodospirillum rubrum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Rhodospirillum rubrum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
above rapid loss of activity, at room temperature 50% of activity after 12 h | Rhodospirillum rubrum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Rhodospirillum rubrum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
(25R)-3beta-hydroxycholest-5-en-27-oate | diisopropylfluorophosphate | Rhodospirillum rubrum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhodospirillum rubrum | isoelectric focusing | - |
9.5 |