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Literature summary for 3.1.1.75 extracted from
- Production of PHA depolymerase A (PhaZ5) from Paucimonas lemoignei in Bacillus subtilis (2002), FEMS Microbiol. Lett., 209, 237-241.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Bacillus subtilis WB800 | Paucimonas lemoignei |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Paucimonas lemoignei | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
SDS-PAGE | Paucimonas lemoignei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly[(R)-3-hydroxybutanoate]n + H2O | Paucimonas lemoignei | - |
poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paucimonas lemoignei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, single chromatography step | Paucimonas lemoignei |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture fluid | - |
Paucimonas lemoignei | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
1 unit defined as the amount of protein required to hydrolyze 1 microg of poly(3-hydroxybutyrate) granules to soluble products per minute, 42000 U/mg | Paucimonas lemoignei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly[(R)-3-hydroxybutanoate]n + H2O | - |
Paucimonas lemoignei | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42000, SDS-PAGE | Paucimonas lemoignei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PhaZ5 | - |
Paucimonas lemoignei |
| PHB depolymerase A | - |
Paucimonas lemoignei |
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