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Literature summary for 3.1.1.75 extracted from

  • Sugiyama, A.; Kobayashi, T.; Shiraki, M.; Saito, T.
    Roles of poly(3-hydroxybutyrate) depolymerase and 3HB-oligomer hydrolase in bacterial PHB metabolism (2004), Curr. Microbiol., 48, 424-427.
    View publication on PubMed
Search for more literature for 3.1.1.75

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Ralstonia pickettii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
 cyclic [(R)-3-hydroxybutanoate]5 cyclic 3-hydroxybutyrate oligomer Acidovorax sp.
0.36
-
 [(R)-3-hydroxybutanoate]6 cyclic 3-hydroxybutyrate oligomer Acidovorax sp.
0.56
-
 [(R)-3-hydroxybutanoate]5 linear 3-hydroxybutyrateoligomer Acidovorax sp.
0.67
-
 [(R)-3-hydroxybutanoate]4 linear 3-hydroxybutyrate oligomer Acidovorax sp.
0.84
-
 cyclic [(R)-3-hydroxybutanoate]3 cyclic 3-hydroxybutyrate oligomer Acidovorax sp.
1.3
-
 [(R)-3-hydroxybutanoate]5 linear 3-hydroxybutyrate oligomer Cupriavidus necator
3.1
-
 [(R)-3-hydroxybutanoate]4 linear 3-hydroxybutyrate oligomer Cupriavidus necator
4.1
-
 [(R)-3-hydroxybutanoate]3 linear 3-hydroxybutyrate oligomer Acidovorax sp.
4.6
-
 cyclic [(R)-3-hydroxybutanoate]5 cyclic 3-hydroxybutyrate oligomer Cupriavidus necator
9.7
-
 [(R)-3-hydroxybutanoate]3 linear 3-hydroxybutyrate oligomer Cupriavidus necator

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Ralstonia pickettii
-
-
extracellular
-
 Acidovorax sp.
-
-
intracellular
-
 Cupriavidus necator 5622
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly[(R)-3-hydroxybutanoate]n + H2O Cupriavidus necator
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O Ralstonia pickettii
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O Acidovorax sp.
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O Ralstonia pickettii T1
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Organism

Organism UniProt Comment Textmining
Acidovorax sp.
-
-
-
Cupriavidus necator
-
-
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-
Ralstonia pickettii
-
-
-
Ralstonia pickettii T1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ralstonia pickettii

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
 Ralstonia pickettii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclic [(R)-3-hydroxybutanoate]3 + H2O
-
 Acidovorax sp. ?
-
 ?
cyclic [(R)-3-hydroxybutanoate]5 + H2O
-
 Cupriavidus necator ?
-
 ?
cyclic [(R)-3-hydroxybutanoate]5 + H2O
-
 Acidovorax sp. ?
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O
-
 Cupriavidus necator poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O
-
 Ralstonia pickettii poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O
-
 Acidovorax sp. poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O linear 3-hydroxbutyrate dimer, trimer, tetramer, pentamer, cyclic 3-hydroxbutyrate trimer, pentamer, hexamer Ralstonia pickettii poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O linear 3-hydroxybutyrate dimer, trimer, tetramer, pentamer, cyclic 3-hydroxybutyrate trimer, pentamer, hexamer Cupriavidus necator poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O linear 3-hydroxybutyrate dimer, trimer, tetramer, pentamer, cyclic 3-hydroxybutyrate trimer, pentamer, hexamer Acidovorax sp. poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O linear 3-hydroxybutyrate dimer, trimer, tetramer, pentamer, cyclic 3-hydroxybutyrate trimer, pentamer, hexamer Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O
-
 Ralstonia pickettii T1 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O linear 3-hydroxbutyrate dimer, trimer, tetramer, pentamer, cyclic 3-hydroxbutyrate trimer, pentamer, hexamer Ralstonia pickettii T1 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
[(R)-3-hydroxybutanoate]3 + H2O
-
 Cupriavidus necator ?
-
 ?
[(R)-3-hydroxybutanoate]3 + H2O
-
 Acidovorax sp. ?
-
 ?
[(R)-3-hydroxybutanoate]4 + H2O
-
 Cupriavidus necator ?
-
 ?
[(R)-3-hydroxybutanoate]4 + H2O
-
 Acidovorax sp. ?
-
 ?
[(R)-3-hydroxybutanoate]5 + H2O
-
 Cupriavidus necator ?
-
 ?
[(R)-3-hydroxybutanoate]5 + H2O
-
 Acidovorax sp. ?
-
 ?
[(R)-3-hydroxybutanoate]6 + H2O
-
 Acidovorax sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
PHBDP
-
 Cupriavidus necator
PHBDP
-
 Ralstonia pickettii
PHBDP
-
 Acidovorax sp.
poly(3-hydroxybutyrate) depolymerase
-
 Cupriavidus necator
poly(3-hydroxybutyrate) depolymerase
-
 Ralstonia pickettii
poly(3-hydroxybutyrate) depolymerase
-
 Acidovorax sp.