Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.75 extracted from

  • Brucato, C.L.; Wong, S.S.
    Extracellular poly(3-hydroxybutyrate) depolymerase from Penicillium funiculosum: General characteristics and active site studies (1991), Arch. Biochem. Biophys., 290, 497-502.
    View publication on PubMed
Search for more literature for 3.1.1.75

General Stability

General Stability Organism
freezing and thawing diminishes activity Talaromyces funiculosus

Inhibitors

Inhibitors Comment Organism Structure
1,2-epoxy-3-(p-nitrophenoxy)propane complete inactivation at 100 mM, slow inactivation process Talaromyces funiculosus
diazo-DL-norleucine methyl ester complete inhibition at 25 mM, pseudo-first-order kinetic Talaromyces funiculosus
diisopropyl fluorophosphate complete inhibition at 500 nM Talaromyces funiculosus
dithiothreitol complete inhibition at 5 mM, reversible by H2O2 Talaromyces funiculosus
Hg2+ 90% inhibition at 6 mM, completely reversible by 2-mercaptoethanol Talaromyces funiculosus
Triton X-100 competitive inhibition, Ki: 0.014% Talaromyces funiculosus
Tween 80 competitive inhibition, Ki: 0.56% Talaromyces funiculosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 poly(3-hydroxybutanoate)n 0.17 mg/ml Talaromyces funiculosus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Talaromyces funiculosus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 gel filtration Talaromyces funiculosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly[(R)-3-hydroxybutanoate]n + H2O Talaromyces funiculosus
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Organism

Organism UniProt Comment Textmining
Talaromyces funiculosus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 12% carbohydrate content by weight Talaromyces funiculosus

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, single chromatography step Talaromyces funiculosus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Talaromyces funiculosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 1 unit defined as the amount of protein required to decrease the value of A650 by 1.0 per minute Talaromyces funiculosus
additional information
-
 51.2 U/mg Talaromyces funiculosus

Storage Stability

Storage Stability Organism
4°C, 10 mM sodium acetate, pH 6.0, 4 weeks Talaromyces funiculosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(3-hydroxybutyric acid) + H2O
-
 Talaromyces funiculosus ?
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O
-
 Talaromyces funiculosus poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 37000, SDS-PAGE Talaromyces funiculosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable below for 15 min Talaromyces funiculosus
50
-
 completely inactive above Talaromyces funiculosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Talaromyces funiculosus

pH Stability

pH Stability pH Stability Maximum Comment Organism
1
-
 inactivation below Talaromyces funiculosus
10
-
 inactivation above Talaromyces funiculosus