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Literature summary for 3.1.1.75 extracted from

  • Asano, Y.; Watanabe, S.
    Isolation of poly(3-hydroxybutyrate) (PHB)-degrading microorganisms and characterization of PHB-depolymerase from Arthrobacter sp. strain W6 (2001), Biosci. Biotechnol. Biochem., 65, 1191-1194.
    View publication on PubMed
Search for more literature for 3.1.1.75

Application

Application Comment Organism
degradation development of biodegradable plastics such as poly(3-hydroxybutyrate) or poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Arthrobacter sp.

General Stability

General Stability Organism
unstable to (NH4)2SO4 treatment Arthrobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ marked inhibition at 0.1 mM Arthrobacter sp.
Hg2+ marked inhibition at 0.1 mM Arthrobacter sp.
Pb2+ marked inhibition at 0.1 mM Arthrobacter sp.
PMSF marked inhibition at 0.1 mM Arthrobacter sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Arthrobacter sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 native PAGE, SDS-PAGE Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly[(R)-3-hydroxybutanoate]n + H2O Arthrobacter sp.
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
 strains W1 and W5, degradation of BIOPOL only, strain W6, degradation of poly[(R)-3-hydroxybutanoate]n, BIOPOL and other polyhydroxyalkanoic acids
-
Pseudomonas alcaligenes
-
 strains W2, W3 and W4, degradation of BIOPOL
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Arthrobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Arthrobacter sp.
-
cell culture
-
 Pseudomonas alcaligenes
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 32.1 mg poly[(R)-3-hydroxybutanoate] /min * mg protein Arthrobacter sp.

Storage Stability

Storage Stability Organism
4°C, culture supernatant, stable up to 20 days Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(3-hydroxybutyrate-co-3-hydroxyvalerate)n + H2O BIOPOL Arthrobacter sp. poly(3-hydroxybutyrate-co-3-hydroxyvalerate)n-x + poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
-
 ?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate)n + H2O BIOPOL Pseudomonas alcaligenes poly(3-hydroxybutyrate-co-3-hydroxyvalerate)n-x + poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
-
 ?
poly(3-hydroxybutyrate-co-[14%]3-hydroxyvalerate)n + H2O
-
 Arthrobacter sp. poly(3-hydroxybutyrate-co-[14%]3-hydroxyvalerate)n-x + poly(3-hydroxybutyrate-co-[14%]3-hydroxyvalerate)
-
 ?
poly(3-hydroxybutyrate-co-[22%]3-hydroxyvalerate)n + H2O best carbon source for enzyme synthesis in strain W6 Arthrobacter sp. poly(3-hydroxybutyrate-co-[22%]3-hydroxyvalerate)n-x + poly(3-hydroxybutyrate-co-[22%]3-hydroxyvalerate)
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O
-
 Arthrobacter sp. poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 47000, SDS-PAGE Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Arthrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable below Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 7 more than 95% of maximal activity Arthrobacter sp.