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Literature summary for 3.1.1.74 extracted from

  • Yang, S.; Liu, M.; Long, L.; Zhang, R.; Ding, S.
    Characterization of a cutinase from Myceliophthora thermophila and its application in polyester hydrolysis and deinking process (2018), Process Biochem., 66, 106-112 .
No PubMed abstract available
Search for more literature for 3.1.1.74

Application

Application Comment Organism
degradation enzyme shows an ink removal efficiency of 78.4% on laser-printed paper and 81.3% on newspaper at 30°C Thermothelomyces thermophilus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Thermothelomyces thermophilus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 82% residual activity Thermothelomyces thermophilus
Ni2+ 1 mM, 74% residual activity Thermothelomyces thermophilus
SDS 1 mM, 16% residual activity Thermothelomyces thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.41
-
 4-nitrophenyl propionate pH 8.5, 30°C Thermothelomyces thermophilus
1.61
-
 4-nitrophenyl pentanoate pH 8.5, 30°C Thermothelomyces thermophilus
2.34
-
 4-nitrophenyl butanoate pH 8.5, 30°C Thermothelomyces thermophilus

Organism

Organism UniProt Comment Textmining
Thermothelomyces thermophilus G2QH51
-
-
Thermothelomyces thermophilus DSM 1799 G2QH51
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butanoate + H2O
-
 Thermothelomyces thermophilus 4-nitrophenol + butanoate
-
 ?
4-nitrophenyl butanoate + H2O
-
 Thermothelomyces thermophilus DSM 1799 4-nitrophenol + butanoate
-
 ?
4-nitrophenyl pentanoate + H2O 53% of the activity with 4-nitrophenyl butanoate Thermothelomyces thermophilus 4-nitrophenol + pentanoate
-
 ?
4-nitrophenyl pentanoate + H2O 53% of the activity with 4-nitrophenyl butanoate Thermothelomyces thermophilus DSM 1799 4-nitrophenol + pentanoate
-
 ?
4-nitrophenyl propionate + H2O 35% of the activity with 4-nitrophenyl butanoate Thermothelomyces thermophilus 4-nitrophenol + propionate
-
 ?
4-nitrophenyl propionate + H2O 35% of the activity with 4-nitrophenyl butanoate Thermothelomyces thermophilus DSM 1799 4-nitrophenol + propionate
-
 ?
cutin + H2O
-
 Thermothelomyces thermophilus ?
-
 ?
cutin + H2O
-
 Thermothelomyces thermophilus DSM 1799 ?
-
 ?
poly(caprolactone) + H2O
-
 Thermothelomyces thermophilus ?
-
 ?
poly(caprolactone) + H2O
-
 Thermothelomyces thermophilus DSM 1799 ?
-
 ?
polyvinyl acetate + H2O substrate used in papermaking and in synthetic toner or ink Thermothelomyces thermophilus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 23400, calculated from sequence, x * 23400, SDS-PAGE Thermothelomyces thermophilus

Synonyms

Synonyms Comment Organism
MYCTH_2110987
-
 Thermothelomyces thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Thermothelomyces thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 30 more than 60% of maximum activity Thermothelomyces thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 1.5 h, 80% residual activity Thermothelomyces thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Thermothelomyces thermophilus

pH Range

pH Minimum pH Maximum Comment Organism
3 11 more than 60% of maximum activity Thermothelomyces thermophilus