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Literature summary for 3.1.1.74 extracted from
- Comparison of genetic structures and biochemical properties of tandem cutinase-type polyesterases from Thermobifida alba AHK119 (2015), J. Biosci. Bioeng., 120, 491-497 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A68V/T253P | increase in both activity and thermostabililty, stable for 1 h below 60°C. Mutant is able to degrade various aliphatic and aliphatic coaromatic polyesters and hydrophilizes an amorphous PET film | Thermobifida alba |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibitory: EDTA up to 10 mM | Thermobifida alba |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 300 mM, about 4fold increase in specific activity. Presence of Ca2+ does not affect the secondary structure of Est1 | Thermobifida alba |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermobifida alba | D4Q9N1 | isoform Est1 | - |
| Thermobifida alba AHK119 | D4Q9N1 | isoform Est1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Thermobifida alba |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.88 | - |
wild-type, pH 7, 37°C | Thermobifida alba |
| 14.5 | - |
wild-type, presence of 300 mM Ca2+, pH 7.0, 37°C | Thermobifida alba |
| 277 | - |
mutant A68V/T253P, pH 7, 37°C | Thermobifida alba |
| 833 | - |
mutant A68V/T253P, presence of 300 mM Ca2+, pH 7.0, 37°C | Thermobifida alba |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl butanoate + H2O | - |
Thermobifida alba | 4-nitrophenol + butanoate | - |
? | |
| 4-nitrophenyl butanoate + H2O | - |
Thermobifida alba AHK119 | 4-nitrophenol + butanoate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
1 h, 70% residual activity, wild-type. In presence of 300 mM Ca2+, no loss of activity for 20 h | Thermobifida alba |
| 79 | - |
melting temperature, mutants A68V/T253P, A68V/T253P/M259K, presence of Ca2+ | Thermobifida alba |
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