Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris. No significant differences between the expression of native Thc_Cut1 and of two glycosylation site knock out mutants (Thc_Cut1_koAsn and Thc_Cut1_koST) concerning the total extracellular protein concentration and volumetric activity are observed | Thermobifida cellulosilytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida cellulosilytica | E9LVH8 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Thermobifida cellulosilytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(ethylene terephthalate)n + H2O | hydrolysis of poly(ethylene terephthalate) (PET) is shown for all three enzymes (native Thc_Cut1 and two glycosylation site knockout mutants (Thc_Cut1_koAsn and Thc_Cut1_koST)) based on quantification of released products by HPLC and similar concentrations of released terephthalic acid (TPA) and mono(2-hydroxyethyl) terephthalate (MHET) | Thermobifida cellulosilytica | terephthalic acid + mono(2-hydroxyethyl)terephthalate + ? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | poly(butylene succinate) is hydrolyzed to significantly higher extent than poly(3-hydroxybutyrate-co-3-hydroxyvalerate) | Thermobifida cellulosilytica | 3-hydroxybutyric acid + ? | - |
? | |
poly(butylene succinate) + H2O | poly(butylene succinate) is hydrolyzed to significantly higher extent than poly(3-hydroxybutyrate-co-3-hydroxyvalerate) | Thermobifida cellulosilytica | succinic acid + 1,4-butanediol + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cutinase 1 | - |
Thermobifida cellulosilytica |