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Literature summary for 3.1.1.74 extracted from

  • Gamerith, C.; Vastano, M.; Ghorbanpour, S.M.; Zitzenbacher, S.; Ribitsch, D.; Zumstein, M.T.; Sander, M.; Herrero Acero, E.; Pellis, A.; Guebitz, G.M.
    Enzymatic degradation of aromatic and aliphatic polyesters by P. pastoris expressed cutinase 1 from Thermobifida cellulosilytica (2017), Front. Microbiol., 8, 938 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris. No significant differences between the expression of native Thc_Cut1 and of two glycosylation site knock out mutants (Thc_Cut1_koAsn and Thc_Cut1_koST) concerning the total extracellular protein concentration and volumetric activity are observed Thermobifida cellulosilytica

Organism

Organism UniProt Comment Textmining
Thermobifida cellulosilytica E9LVH8
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Thermobifida cellulosilytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(ethylene terephthalate)n + H2O hydrolysis of poly(ethylene terephthalate) (PET) is shown for all three enzymes (native Thc_Cut1 and two glycosylation site knockout mutants (Thc_Cut1_koAsn and Thc_Cut1_koST)) based on quantification of released products by HPLC and similar concentrations of released terephthalic acid (TPA) and mono(2-hydroxyethyl) terephthalate (MHET) Thermobifida cellulosilytica terephthalic acid + mono(2-hydroxyethyl)terephthalate + ?
-
?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O poly(butylene succinate) is hydrolyzed to significantly higher extent than poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Thermobifida cellulosilytica 3-hydroxybutyric acid + ?
-
?
poly(butylene succinate) + H2O poly(butylene succinate) is hydrolyzed to significantly higher extent than poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Thermobifida cellulosilytica succinic acid + 1,4-butanediol + ?
-
?

Synonyms

Synonyms Comment Organism
cutinase 1
-
Thermobifida cellulosilytica