Literature summary for 3.1.1.74 extracted from

Shirke, A.N.; Butterfoss, G.L.; Saikia, R.; Basu, A.; de Maria, L.; Svendsen, A.; Gross, R.A.
Engineered Humicola insolens cutinase for efficient cellulose acetate deacetylation (2017), Biotechnol. J., 12, 1700188 .

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Humicola insolens

Protein Variants

Protein Variants	Comment	Organism
I36N/F70S	mutant engineered for cellulose acetate deacetylation, almost 2fold improvement in catalytic efficiency with both cellulose acetate and 4-nitrophenyl butanoate	Humicola insolens
I36S/F70A	mutant engineered for cellulose acetate deacetylation, 2fold improvement in catalytic efficiency with cellulose acetate and 4-nitrophenyl butanoate	Humicola insolens

Organism

Organism	UniProt	Comment	Textmining
Humicola insolens	A0A075B5G4	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	enzyme additionally shows cellulose acetate deacylation activity	Humicola insolens	?	-	?

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Release 2023.1 (January 2023)