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Literature summary for 3.1.1.74 extracted from

  • Duan, X.; Liu, Y.; You, X.; Jiang, Z.; Yang, S.; Yang, S.
    High-level expression and characterization of a novel cutinase from Malbranchea cinnamomea suitable for butyl butyrate production (2017), Biotechnol. Biofuels, 10, 223 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.74

Activating Compound

Activating Compound Comment Organism Structure
SDS 5%, 131% of initial activity Malbranchea cinnamomea
Triton X-100 5%, 190% of initial activity Malbranchea cinnamomea
Tween 20 5%, 118% of initial activity Malbranchea cinnamomea
Tween 40 5%, 139.7% of initial activity Malbranchea cinnamomea
Tween 60 5%, 139.5% of initial activity Malbranchea cinnamomea
Tween 80 5%, 141.9% of initial activity Malbranchea cinnamomea

Application

Application Comment Organism
synthesis enzyme synthesizes butyl butanoate with a maximum esterification efficiency of 96.9% at 4 h Malbranchea cinnamomea

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Malbranchea cinnamomea

Crystallization (Commentary)

Crystallization (Comment) Organism
structure at 1.76 A resolution in space group P21221 Malbranchea cinnamomea

Organic Solvent Stability

Organic Solvent Comment Organism
acetonitrile 30%, 93.1% residual activity Malbranchea cinnamomea
dimethyl sulfoxide 30%, 80.9% residual activity Malbranchea cinnamomea
Ethanol 30%, 72.8 % residual activity Malbranchea cinnamomea
hexane 30%, 97.7% residual activity Malbranchea cinnamomea
Methanol 30%, 80% residual activity Malbranchea cinnamomea

Organism

Organism UniProt Comment Textmining
Malbranchea cinnamomea A0A1S6YJF3
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1148
-
 pH 8.0, 50°C, substrate 4-nitrophenyl butanoate Malbranchea cinnamomea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butanoate + H2O
-
 Malbranchea cinnamomea 4-nitrophenol + butanoate
-
 ?
4-nitrophenyl hexanoate + H2O
-
 Malbranchea cinnamomea 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl myristate + H2O
-
 Malbranchea cinnamomea 4-nitrophenol + myristate
-
 ?
butanol + butanoate
-
 Malbranchea cinnamomea butyl butanoate + H2O
-
 ?
cutin + H2O
-
 Malbranchea cinnamomea cutin monomers
-
 ?
poly(butylene succinaste) + H2O
-
 Malbranchea cinnamomea ?
-
 ?
poly(caprolactone) + H2O
-
 Malbranchea cinnamomea ?
-
 ?
tributyrin + H2O
-
 Malbranchea cinnamomea ?
-
 ?

Subunits

Subunits Comment Organism
? x * 21180, calculated from sequence Malbranchea cinnamomea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Malbranchea cinnamomea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
 stable up to Malbranchea cinnamomea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Malbranchea cinnamomea

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10.5
-
 Malbranchea cinnamomea

pI Value

Organism Comment pI Value Maximum pI Value
Malbranchea cinnamomea calculated from sequence
-
 5.2