Literature summary for 3.1.1.74 extracted from

Dimarogona, M.; Nikolaivits, E.; Kanelli, M.; Christakopoulos, P.; Sandgren, M.; Topakas, E.
Structural and functional studies of a Fusarium oxysporum cutinase with polyethylene terephthalate modification potential (2015), Biochim. Biophys. Acta, 1850, 2308-2317 .

Search for more literature for 3.1.1.74

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Fusarium oxysporum

Crystallization (Commentary)

Crystallization (Comment)	Organism
modeling of structure	Fusarium oxysporum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.7	-	4-nitrophenyl butanoate	pH 6.0, 40°C	Fusarium oxysporum
2.1	-	4-nitrophenyl laurate	pH 6.0, 40°C	Fusarium oxysporum
5.4	-	4-nitrophenyl acetate	pH 6.0, 40°C	Fusarium oxysporum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	recombinant protein	Fusarium oxysporum	5737	-
periplasm	recombinant protein	Fusarium oxysporum	-	-

Organism

Organism	UniProt	Comment	Textmining
Fusarium oxysporum	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl acetate + H2O	-	Fusarium oxysporum	4-nitrophenol + acetate	-	?
4-nitrophenyl butanoate + H2O	-	Fusarium oxysporum	4-nitrophenol + butanoate	-	?
4-nitrophenyl laurate	-	Fusarium oxysporum	4-nitrophenol + laurate	-	?
additional information	the enzyme is capable of hydrolyzing polyethylene terephthalate model substrates and synthetic polymers	Fusarium oxysporum	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 231740, calculated from sequence, x * 23000, SDS-PAGE	Fusarium oxysporum

Synonyms

Synonyms	Comment	Organism
Cut5a	-	Fusarium oxysporum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Fusarium oxysporum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
8.3	-	4-nitrophenyl laurate	pH 6.0, 40°C	Fusarium oxysporum
111.9	-	4-nitrophenyl butanoate	pH 6.0, 40°C	Fusarium oxysporum
121	-	4-nitrophenyl acetate	pH 6.0, 40°C	Fusarium oxysporum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Fusarium oxysporum

pI Value

Organism	Comment	pI Value Maximum	pI Value
Fusarium oxysporum	calculated from sequence	-	8.4

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
3.9	-	4-nitrophenyl laurate	pH 6.0, 40°C	Fusarium oxysporum
22.5	-	4-nitrophenyl acetate	pH 6.0, 40°C	Fusarium oxysporum
152.5	-	4-nitrophenyl butanoate	pH 6.0, 40°C	Fusarium oxysporum

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Release 2023.1 (January 2023)