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Literature summary for 3.1.1.74 extracted from

  • Su, L.; Chen, S.; Yi, L.; Woodard, R.W.; Chen, J.; Wu, J.
    Extracellular overexpression of recombinant Thermobifida fusca cutinase by alpha-hemolysin secretion system in E. coli BL21(DE3) (2012), Microb. Cell Fact., 11, 8-8.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.74

Application

Application Comment Organism
industry the enzyme is mainly utilized in textile industry Thermobifida fusca

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression via type II secretory system in Escherichia coli strain BL21(DE3), the protein accumulates in the periplasmic space. The alpha-hemolysin secretion system can export target proteins directly from cytoplasm across both cell membrane of Escherichia coli to the culture medium and is therefore used for expression of the extracellular enzyme, method optimization for large-scale industrial production, overview Thermobifida fusca

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Thermobifida fusca
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Thermobifida fusca cutinase is a multi-functional esterase, which shows hydrolytic activity (cutin and a variety of soluble synthetic esters, insoluble triglycerides and polyesters), synthetic activity and transester activity ?
-
 ?

Organism

Organism UniProt Comment Textmining
Thermobifida fusca
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant extracellular enzyme 2.0fold from culture supernatant by ammonium sulfate precipitation and two different steps of anion exchange chromatography Thermobifida fusca

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
446.2
-
 purified recombinant alpha-hemolysin-enzyme, substrate 4-nitrophenyl butyrate, pH 8.0, 50°C Thermobifida fusca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butyrate + H2O
-
 Thermobifida fusca 4-nitrophenol + butyrate
-
 ?
cutin + H2O
-
 Thermobifida fusca cutin monomers reaction products include hexadecanoic acid, octadecanoic acid, 9-octadecenoic acid, 9,12-octadecadienoic acid, 16-hydroxy hexadecanoic acid, and 18-hydroxyoctadeca-9,12-dienoic acid, ratios of wild-type and recombinant alpha-hemolysin-enzyme differ, overview ?
additional information cutinase is a multi-functional esterase, which shows hydrolytic activity (cutin and a variety of soluble synthetic esters, insoluble triglycerides and polyesters), synthetic activity and transester activity Thermobifida fusca ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 wild-type enzyme Thermobifida fusca
70
-
 recombinant alpha-hemolysin-enzyme Thermobifida fusca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 purified recombinant alpha-hemolysin-cutinase retains more than 90% of its maximal activity after incubation at pH 4.0-11.0 for 24 h, which is superior to that of wild-type cutinase Thermobifida fusca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 recombinant alpha-hemolysin-enzyme Thermobifida fusca

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 11 purified recombinant alpha-hemolysin-cutinase retains more than 90% of its maximal activity after incubation at 37°C for 24 h in this pH range, which is superior to that of wild-type cutinase Thermobifida fusca