Cloned (Comment) | Organism |
---|---|
high-level overexpression of Glomerella cingulata cutinase, with a cutinase production of 3800 mg/l and an activity of 434 U/ml, in dense cultures of Pichia pastoris strain X-33 grown under fed-batch conditions, method evaluation, overview | Colletotrichum gloeosporioides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | slight inhibition at 1 mM | Colletotrichum gloeosporioides | |
Mg2+ | slight inhibition at 1 mM | Colletotrichum gloeosporioides | |
Mn2+ | slight inhibition at 1 mM | Colletotrichum gloeosporioides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Colletotrichum gloeosporioides | |
7.14 | - |
4-nitrophenyl laurate | pH 8.0, 25°C, recombinant enzyme | Colletotrichum gloeosporioides | |
7.24 | - |
4-nitrophenyl caprylate | pH 8.0, 25°C, recombinant enzyme | Colletotrichum gloeosporioides | |
7.25 | - |
4-nitrophenyl myristate | pH 8.0, 25°C, recombinant enzyme | Colletotrichum gloeosporioides | |
20 | - |
4-nitrophenyl butyrate | pH 8.0, 25°C, recombinant enzyme | Colletotrichum gloeosporioides | |
63 | - |
4-nitrophenyl acetate | pH 8.0, 25°C, recombinant enzyme | Colletotrichum gloeosporioides |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight activation at 1 mM | Colletotrichum gloeosporioides | |
additional information | the enzyme activity is poorly or not affected by 1 mM Li+, K+, Co2+, and Zn2+ | Colletotrichum gloeosporioides | |
Na+ | slight activation at 1 mM | Colletotrichum gloeosporioides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 25000, SDS-PAGE | Colletotrichum gloeosporioides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Colletotrichum gloeosporioides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme partially 293fold from Pichia pastoris strain X-33 by ultrafiltration | Colletotrichum gloeosporioides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
114 | - |
purified recombinant enzyme, pH 8.0, 25°C | Colletotrichum gloeosporioides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | low activity | Colletotrichum gloeosporioides | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | low activity | Colletotrichum gloeosporioides | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl caprylate + H2O | high activity | Colletotrichum gloeosporioides | 4-nitrophenol + caprylate | - |
? | |
4-nitrophenyl laurate + H2O | high activity | Colletotrichum gloeosporioides | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl myristate + H2O | high activity | Colletotrichum gloeosporioides | 4-nitrophenol + myristate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, SDS-PAGE | Colletotrichum gloeosporioides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Colletotrichum gloeosporioides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant enzyme, pH 8.0, 30 min, stable at | Colletotrichum gloeosporioides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Colletotrichum gloeosporioides |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | recombinant enzyme, 25°C, stable at | Colletotrichum gloeosporioides |
Organism | Comment | Expression |
---|---|---|
Colletotrichum gloeosporioides | the recombinant enzyme is induced with 0.5% v/v methanol in basal salt medium at pH 5.0 and 28°C | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
4-nitrophenyl acetate | pH 8.0, 25°C, recombinant enzyme | Colletotrichum gloeosporioides | |
2.2 | - |
4-nitrophenyl butyrate | pH 8.0, 25°C, recombinant enzyme | Colletotrichum gloeosporioides | |
6.14 | - |
4-nitrophenyl laurate | pH 8.0, 25°C, recombinant enzyme | Colletotrichum gloeosporioides | |
6.4 | - |
4-nitrophenyl myristate | pH 8.0, 25°C, recombinant enzyme | Colletotrichum gloeosporioides | |
7.7 | - |
4-nitrophenyl caprylate | pH 8.0, 25°C, recombinant enzyme | Colletotrichum gloeosporioides |