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Literature summary for 3.1.1.74 extracted from

  • Seman, W.M.; Bakar, S.A.; Bukhari, N.A.; Gaspar, S.M.; Othman, R.; Nathan, S.; Mahadi, N.M.; Jahim, J.; Murad, A.M.; Bakar, F.D.
    High level expression of Glomerella cingulata cutinase in dense cultures of Pichia pastoris grown under fed-batch conditions (2014), J. Biotechnol., 184, 219-228.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
high-level overexpression of Glomerella cingulata cutinase, with a cutinase production of 3800 mg/l and an activity of 434 U/ml, in dense cultures of Pichia pastoris strain X-33 grown under fed-batch conditions, method evaluation, overview Colletotrichum gloeosporioides

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ slight inhibition at 1 mM Colletotrichum gloeosporioides
Mg2+ slight inhibition at 1 mM Colletotrichum gloeosporioides
Mn2+ slight inhibition at 1 mM Colletotrichum gloeosporioides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Colletotrichum gloeosporioides
7.14
-
4-nitrophenyl laurate pH 8.0, 25°C, recombinant enzyme Colletotrichum gloeosporioides
7.24
-
4-nitrophenyl caprylate pH 8.0, 25°C, recombinant enzyme Colletotrichum gloeosporioides
7.25
-
4-nitrophenyl myristate pH 8.0, 25°C, recombinant enzyme Colletotrichum gloeosporioides
20
-
4-nitrophenyl butyrate pH 8.0, 25°C, recombinant enzyme Colletotrichum gloeosporioides
63
-
4-nitrophenyl acetate pH 8.0, 25°C, recombinant enzyme Colletotrichum gloeosporioides

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slight activation at 1 mM Colletotrichum gloeosporioides
additional information the enzyme activity is poorly or not affected by 1 mM Li+, K+, Co2+, and Zn2+ Colletotrichum gloeosporioides
Na+ slight activation at 1 mM Colletotrichum gloeosporioides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
x * 25000, SDS-PAGE Colletotrichum gloeosporioides

Organism

Organism UniProt Comment Textmining
Colletotrichum gloeosporioides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme partially 293fold from Pichia pastoris strain X-33 by ultrafiltration Colletotrichum gloeosporioides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
114
-
purified recombinant enzyme, pH 8.0, 25°C Colletotrichum gloeosporioides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O low activity Colletotrichum gloeosporioides 4-nitrophenol + acetate
-
?
4-nitrophenyl butyrate + H2O low activity Colletotrichum gloeosporioides 4-nitrophenol + butyrate
-
?
4-nitrophenyl caprylate + H2O high activity Colletotrichum gloeosporioides 4-nitrophenol + caprylate
-
?
4-nitrophenyl laurate + H2O high activity Colletotrichum gloeosporioides 4-nitrophenol + laurate
-
?
4-nitrophenyl myristate + H2O high activity Colletotrichum gloeosporioides 4-nitrophenol + myristate
-
?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Colletotrichum gloeosporioides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Colletotrichum gloeosporioides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
recombinant enzyme, pH 8.0, 30 min, stable at Colletotrichum gloeosporioides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Colletotrichum gloeosporioides

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11 recombinant enzyme, 25°C, stable at Colletotrichum gloeosporioides

Expression

Organism Comment Expression
Colletotrichum gloeosporioides the recombinant enzyme is induced with 0.5% v/v methanol in basal salt medium at pH 5.0 and 28°C up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.48
-
4-nitrophenyl acetate pH 8.0, 25°C, recombinant enzyme Colletotrichum gloeosporioides
2.2
-
4-nitrophenyl butyrate pH 8.0, 25°C, recombinant enzyme Colletotrichum gloeosporioides
6.14
-
4-nitrophenyl laurate pH 8.0, 25°C, recombinant enzyme Colletotrichum gloeosporioides
6.4
-
4-nitrophenyl myristate pH 8.0, 25°C, recombinant enzyme Colletotrichum gloeosporioides
7.7
-
4-nitrophenyl caprylate pH 8.0, 25°C, recombinant enzyme Colletotrichum gloeosporioides