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Literature summary for 3.1.1.74 extracted from

  • Liu, Z.; Gosser, Y.; Baker, P.J.; Ravee, Y.; Lu, Z.; Alemu, G.; Li, H.; Butterfoss, G.L.; Kong, X.P.; Gross, R.; Montclare, J.K.
    Structural and functional studies of Aspergillus oryzae cutinase: enhanced thermostability and hydrolytic activity of synthetic ester and polyester degradation (2009), J. Am. Chem. Soc., 131, 15711-15716.
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
biotechnology engineering new cutinase-inspired biocatalysts with tailor-made properties Aspergillus oryzae
biotechnology engineering new cutinase-inspired biocatalysts with tailor-made properties Fusarium solani

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Fusarium solani

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Fusarium solani
to 1.75 A resolution. Alpha/beta fold hallmarked by a central beta-sheet of 5 parallel strands surrounded by 10 alpha-helices. An additional disulfide bond and a topologically favored catalytic triad (Ser126, Asp181, and His194), with a continuous and deep groove Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00004
-
 4-nitrophenyl valerate in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol Aspergillus oryzae
0.00021
-
 4-nitrophenyl butyrate in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol Aspergillus oryzae
0.00029
-
 4-nitrophenyl hexanoate in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol Aspergillus oryzae
0.00067
-
 4-nitrophenyl acetate in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol Fusarium solani
0.00126
-
 4-nitrophenyl butyrate in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol Fusarium solani
0.00148
-
 4-nitrophenyl valerate in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol Fusarium solani
0.0015
-
 4-nitrophenyl hexanoate in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol Fusarium solani
0.00496
-
 4-nitrophenyl acetate in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-
Fusarium solani
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by centrifugation, ultrafiltration and on Ni2+ column Fusarium solani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Aspergillus oryzae 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O
-
 Fusarium solani 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Aspergillus oryzae 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Fusarium solani 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl hexanoate
-
 Aspergillus oryzae 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl hexanoate
-
 Fusarium solani 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl valerate
-
 Aspergillus oryzae 4-nitrophenol + pentanoate
-
 ?
4-nitrophenyl valerate
-
 Fusarium solani 4-nitrophenol + pentanoate
-
 ?

Subunits

Subunits Comment Organism
monomer crystallography Aspergillus oryzae

Synonyms

Synonyms Comment Organism
cutinase
-
 Aspergillus oryzae
cutinase
-
 Fusarium solani

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
56
-
 melting temperature. Dramatic loss in activity at 40 °C, 40% drop at 30 °C, continuous decline in activity as a function of increasing temperature Fusarium solani
59
-
 melting temperature. Maintains a high level of activity at 40 °C Aspergillus oryzae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00000043
-
 4-nitrophenyl butyrate in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol Fusarium solani
0.00000167
-
 4-nitrophenyl acetate in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol Aspergillus oryzae
0.0000023
-
 4-nitrophenyl hexanoate in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol Fusarium solani
0.00001017
-
 4-nitrophenyl valerate in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol Fusarium solani
0.000023
-
 4-nitrophenyl hexanoate in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol Aspergillus oryzae
0.00004217
-
 4-nitrophenyl acetate in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol Fusarium solani
0.000053
-
 4-nitrophenyl valerate in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol Aspergillus oryzae
0.0000582
-
 4-nitrophenyl butyrate in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol Aspergillus oryzae