Cloned (Comment) | Organism |
---|---|
overexpression of recombinant cutinase and its variants is performed with strain Escherichia coli WK-6 (delta(lac-pro) galE strA F'[lacIq ZdeltaM15 proAB+]) | Fusarium solani |
Protein Variants | Comment | Organism |
---|---|---|
A164E | amino acid substitution, 74% of wild-type activity | Fusarium solani |
A185T | amino acid substitution, 142% of wild-type activity | Fusarium solani |
G26P | amino acid substitution, 68% of wild-type activity | Fusarium solani |
G41A | amino acid substitution, 76% of wild-type activity | Fusarium solani |
K140D | amino acid substitution, 39% of wild-type activity | Fusarium solani |
K151M | amino acid substitution, 22% of wild-type activity | Fusarium solani |
K65P | amino acid substitution, 99% of wild-type activity | Fusarium solani |
L153A | amino acid substitution, 111% of wild-type activity | Fusarium solani |
L153Q | amino acid substitution, 145% of wild-type activity | Fusarium solani |
additional information | a complete saturation mutagenesis approach to search cutinase for amino acids contributing to increased stability in the presence of the anionic surfactant. Mutants showing substitutions in the large hydrophobic crevice (S54D, S57D, S61D, K65P, R196A), that is thought to be the region more involved in the unfolding by anionics, will be very important to obtain an enzyme less sensitive to AOT | Fusarium solani |
R156K | amino acid substitution, 151% of wild-type activity | Fusarium solani |
R156N | amino acid substitution, 89% of wild-type activity | Fusarium solani |
R158L | amino acid substitution, 75% of wild-type activity | Fusarium solani |
R17S | amino acid substitution, 69% of wild-type activity | Fusarium solani |
R196A | amino acid substitution, 75% of wild-type activity | Fusarium solani |
R196E | amino acid substitution, 21% of wild-type activity | Fusarium solani |
S54D | amino acid substitution, 79% of wild-type activity | Fusarium solani |
S54E | amino acid substitution, 83% of wild-type activity | Fusarium solani |
S57D | amino acid substitution, 61% of wild-type activity | Fusarium solani |
S61D | amino acid substitution, 83% of wild-type activity | Fusarium solani |
T179C | amino acid substitution, 90% of wild-type activity | Fusarium solani |
T179E | amino acid substitution, 10% of wild-type activity | Fusarium solani |
T179N | amino acid substitution, 119% of wild-type activity | Fusarium solani |
T18D | amino acid substitution, 65% of wild-type activity | Fusarium solani |
T45D | amino acid substitution, 54% of wild-type activity | Fusarium solani |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Fusarium solani | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium solani | - |
pisi | - |
Purification (Comment) | Organism |
---|---|
- |
Fusarium solani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Fusarium solani | 4-nitrophenol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cutinase | - |
Fusarium solani |